【摘要】 目的:研究有氧运动对衰老模型大鼠骨骼肌细胞凋亡及Bcl-2和Bax蛋白表达的影响。方法:建立衰老大鼠模型,随机分为对照组和2组运动组,运动组分别给予6次/周和3次(隔日)/周的游泳训练,每次运动持续90 min,共训练12周,于末次训练48 h后处死动物。TUNEL法检测骨骼肌细胞凋亡,免疫印迹法检测骨骼肌细胞内Bcl-2、Bax的表达,并计算Bcl-2/Bax的比值。结果:与对照组相比,2组运动组骨骼肌细胞凋亡指数和Bax蛋白表达均显著降低(P均<0.01),而Bcl-2蛋白表达和Bcl-2/Bax明显升高(P均<0.01);与6次/周运动组比较,3次/周运动组骨骼肌凋亡指数和Bax表达明显降低(P均<0.05),而Bcl-2表达和Bcl-2/Bax明显升高(P均<0.05)。结论:有氧运动能优化Bcl-2/Bax的比值,降低骨骼肌细胞凋亡指数,有效减少衰老导致的骨骼肌过度凋亡;且隔日有氧运动效果更佳。更多还原

【Abstract】 Objective: To investigate the effect of aerobic exercise on cell apoptosis and expressions of Bcl-2 and Bax protein in skeletal muscle from aging model rats.Methods: The aging model rats were randomly divided into control group and two exercise groups which include 6 times/wk exercise group and 3 times/wk exercise group.Exercise groups were trained by swimming for 12 weeks,90 min each time.All rats were killed after 48 hours of the last training session.Cell apoptosis of skeletal muscle was detected by using TUNEL staining.The expressions of Bcl-2 and Bax in skeletal muscle were quantified by western blotting.Results: Compared with control group,the apoptotic index(AI) of skeletal muscle and expression of Bax protein in exercise groups were obviously decreased(both P<0.01),while the expression of Bcl-2 protein and the ratio of Bcl-2/Bax were markedly increased(both P<0.01).Compared with 6 times/wk group,the AI of skeletal muscle and expression of Bax protein in 3 times/wk group were significantly decreased(P<0.05),whereas the expression of Bcl-2 protein and the ratio of Bcl-2/Bax were greatly increased(P<0.05).Conclusion: The ratio of Bcl-2/Bax could be increased by aerobic exercise.And the excessive apoptosis of skeletal muscle of aged rats could be reduced by aerobic exercise.The effect of every-other-day aerobic exercise was better than everyday aerobic exercise.It was beneficial to inhibit cell apoptosis of skeletal muscle.更多还原