【摘要】 目的探讨胃癌组织中自噬相关蛋白Beclin1与微管相关蛋白轻链3(MAPLC3)的表达及其临床意义。方法采用免疫组化En-Vision法检测59例胃癌组织和相应的癌旁组织中Beclin1及MAPLC3的表达水平,分析其与多种胃癌临床病理参数之间的关系。结果在胃癌组织中Beclin1和MAPLC3的阳性表达率分别为40.7%和44.1%,癌旁组织的阳性表达率分别为100%、93.2%,癌组织明显低于癌旁组织(P<0.05);Beclin1和MAPLC3的表达水平与胃癌的浸润深度、淋巴结转移及TNM分期相关(P<0.05);胃癌组织中Beclin1和MAPLC3的表达水平呈正相关(r=0.425,P<0.05)。结论胃癌组织中Beclin1和MAPLC3表达水平均明显低于癌旁组织,且两者之间呈正相关,两者的低表达可能共同促进胃癌的淋巴结转移。更多还原

【Abstract】 Objective To investigate the expression of Beclini and MAPLC3 in gastric cancer and clinical significance.Methods Immunohistochemical assay was performed to detect the expression of Beclini and MAPLC3 in fifty-nine gastric carcinoma tissues and their adjacent noncancerous specimens.Results The expression of Beclini and MAPLC3 was both decreased in carcinoma tissues(40.7%and 44.1%),which were significantly lower than those in noncancerous tissues(P<0.05).The expression of both Beclini and MAPLC3 was correlated with depth of invasion,lymph node metastasis and TNM classification(P<0.05).There was positive correlation between the expression of Beclini and MAPLC3(r=0.425,P<0.05).Conclusion The expression of Beclini and MAPLC3 both reduced in gastric carcinoma tissues,and the downregulation of Beclini is significantly associated with MAPLC3.The co-downregulation of Beclini and MAPLC3 may play an important role in lymphatic spread of gastric carcinoma.更多还原