【摘要】 目的探讨鼻咽癌组织中p15^INK4b和p27^KIP1基因启动子区甲基化状态在鼻咽癌临床早期诊断和治疗中的作用。方法应用甲基化特异性PCR方法对65例鼻咽癌组织（鼻咽癌组）和30例正常鼻咽上皮组织（对照组）中的p15^INK4b和p27^KIP1基因启动子区甲基化状态进行检测。结果鼻咽癌组中p15^INK4b和p27^KIP1基因启动子区甲基化阳性率分别为26.2%（17/65）和61.5%（40/65）,两者总检出率为72.3%（47/65）;而对照组中均未检测到p15^INK4b和p27^KIP1基因启动子区甲基化;两组间p15^INK4b和p27^KIP1基因启动子区甲基化阳性率的差异具有统计学意义（均P<0.01）。鼻咽癌组中p15^INK4b和p27^KIP1基因启动子区甲基化与其临床分期及病理特征均无明显相关性（均P>0.05）。同时,p15^INK4b基因甲基化和p27^KIP1,基因甲基化间无明显相关性（r=-0.03,P>0.05）。结论p15^INK4b和p27^KIP1基因启动子区甲基化可能参与鼻咽癌的发生、发展;p15^INK4b和p27^KIP1基因启动子区甲基化具有一定的肿瘤特异性,有助于鼻咽癌的早期辅助诊断和治疗。更多还原

【Abstract】 Objective To investigate the promoter methylation status of p15^INK4b and p27^KIP1 genes in nasopharyngeal carcinoma（NPC）.Methods The methylation status of p15^INK4b and p27^KIP1 genes promoter region were detected by methylation-specific polymerase chain reaction（MSP） in 65 specimens of NPC tissues（NPC group） and 30 specimens of normal nasopharyngeal epithelia tissues（control group）.Results The promoter methylation rates of p15^INK4b and p27^KIP1 genes were 26.2%（17/65） and 61.5%（40/65）,respectively in NPC group,while no methylation of the two genes were detected in control group（P<0.01）.The methylation of p15^INK4b and p27^KIP1 genes were not correlated with the clinicopathological characteristics of patients with NPC（P >0.05）.There were no significant correlation between the methylations of p15^INK4b genes and p27^KIP1 genes（r=-0.03,P >0.05）.Conclusion The methylation of p15^INK4b and p27^KIP1 genes promoter regions may be associated with the development of NPC,which may used as potential markers for early diagnosis of NPC.更多还原