【摘要】 目的:探索肌肽对小鼠闭合性脑损伤的保护作用。方法:建立自由落体法致小鼠闭合性脑损伤模型。从术前30 min开始,二肌肽组经腹腔分别注射肌肽250 mg/kg和500 mg/kg剂量,溶剂对照组及假手术组腹腔注射等量生理盐水。小鼠闭合性损伤后每日1次给药,连续实验7 d,并每天进行神经受损程度评分(NSS),记录小鼠生存率及体重改变。实验结束后取小鼠脑组织,切片、HE染色。结果:溶剂对照组、250 mg/kg肌肽组和500 mg/kg肌肽组小鼠闭合性脑损伤7 d后的最终存活率分别为50.0%、68.2%和82.4%;与溶剂对照组比较,250 mg/kg肌肽可显著降低小鼠闭合性脑损伤术后第5～7天的NSS(P<0.05),500 mg/kg肌肽可显著降低术后第1天及第4～7天的NSS(P<0.05);小鼠脑组织切片结果提示肌肽可显著减轻脑组织的组织病理学损伤。结论:肌肽对小鼠闭合性脑外伤具有保护作用,能提高损伤后小鼠生存率并促进神经功能的恢复。更多还原

【Abstract】 Objective: To investigate the protective effects of carnosine against experimental closed head injury(CHI) in mice.Methods: The CHI model was established by free-falling weight-drop.Carnosine(250 mg/kg or 500 mg/kg) was administered intraperitoneally 30 min before brain trauma,then q.d for 7 d;while normal saline was administrated for control group.The neurological defect was evaluated by neurological severity score(NSS) within 7 d;the survival rate and the histological alternations were observed.Results: Carnosine prevented the body weight loss of mice at dose of 500 mg/kg;significantly increased the survival rate,and reduced the neurological defect and histological damage at dose of 250 and 500 mg/kg.Conclusion: Carnosine can attenuate closed head injury in mice.更多还原