【摘要】 目的分析新生隐球菌荚膜多糖GXM对小鼠神经小胶质细胞能量代谢和凋亡的影响。方法①采用紫外分光光度计检测GXM干预后的神经小胶质细胞能量产物ATP含量的改变情况。②采用AnnexinV-异硫氰酸荧光素(fluorescein isothiocyanate,FITC)/碘化丙啶(propidium iodide,PI)双标记法流式细胞术检测GXM细胞诱导神经小胶质细胞凋亡的情况。结果①GXM体外可诱导神经小胶质细胞产ATP能力下降。②GXM体外可诱导神经小胶质细胞的凋亡。结论新生隐球菌可通过GXM诱导能量代谢紊乱和凋亡来对神经小胶质细胞产生影响。更多还原

【Abstract】 Objective To detect the effect of Cryptococcus neoformans GXM on energy metabolism and apoptosis of mouse microglia cell. Method ①The capacity of ATP generation in each group was evaluated with UV spectrophotometric determination. ②Annexin V-fluorescein isothiocyanate / propidium iodide double label method was taken to detect apoptosis rate of BV-2 cells after co-incubation with GXM. Results ①The capacity of ATP generation decreased after intervention by GXM. ②GXM was able to induce apoptosis in microglial cells in vitro. Conclusion Cryptococcus neoformans could exert effect on host cells via induction of energy metabolism disorder and apoptosis in microglia by GXM.更多还原