【摘要】 [目的]观察不同浓度白细胞介素1β对组织工程化椎间盘细胞基质金属蛋白酶13表达的影响。[方法]将体外培养的兔组织工程化椎间盘细胞随机分为1、10、100μg/L浓度白细胞介素1β作用组及空白对照组,免疫组织化学检测基质金属蛋白酶13表达。[结果]1、10、100μg/L浓度白细胞介素1β组基质金属蛋白酶13阳性表达率均较对照组增高(P<0.05)。随着白细胞介素1β浓度的增高,基质金属蛋白酶13阳性表达率显著升高,100μg/L浓度高于1、10μg/L浓度组(P<0.05),10μg/L浓度组高于1μg/L浓度组(P<0.05)。[结论]白细胞介素1β对兔组织工程化椎间盘细胞基质金属蛋白酶13表达的调控作用随其浓度的升高而呈正相关。更多还原

【Abstract】 [Objective]To observe the effects of different concentrations of interleukin - 18 on the expression of matrix metalloproteinase 13 in tissue - engineered intervertebral disc cell of rabbit.[Methods]Tissue - engineered intervertebral disc cells were cultured with 1,10,100μg / L concentrations of interleukin - 1βand were divided into three groups,the cells were cultured with ordinary medium as the control group,matrix metalloproteinase - 13 expression were observed in vitro by immunohistochemical staining.[Results]1,10,100μg/L interleukin - 1βgroup matrix metalloproteinase 13 positive expression rate increased significantly(P<0.05) than in the control group.With increased interleukin -1βconcentration,matrix metalloproteinase 13 positive expression rate significantly increased(P<0.05),and the differences among groups were significant(P<0.05).[Conclusion]Matrix metalloproteinase 13 expression positively correlated with the increasing concentration of interleukin -1βcultured on rabbit engineered intervertebral disc cells.更多还原