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杨梅花粉活力检测及储藏方法研究
Vitality test and storage method for bayberry pollen
【摘要】 对杨梅(Myrica rubra Sieb.&Zucc.)花粉活力检测和储藏方法进行了研究。筛选出最佳的杨梅花粉活力离体萌发检测法:用毛笔轻刷花粉至固体培养基(15%蔗糖,0.01%硼酸,1%琼脂)上,22℃黑暗培养10h,显微观测花粉萌发率。20℃储藏时,经硅胶干燥处理能延长杨梅花粉活力保存时间。与20℃相比,低温保存能明显延长杨梅花粉活力,在4,-20和-80℃保存100 d后,花粉萌发率分别是12%,37.5%和48%。
【Abstract】 In order to find the best storage method for pollen of bayberry(Myrica rubra Sieb.& Zucc.),the study on the optimal examination method for vitality of bayberry pollen was carried out.The results showed that bayberry pollen germinated best on the solid medium containing 15% sucrose,0.01% boric acid and 1% agar in darkness at 22℃ for 10 h.The silica gel drying could extend the living time of bayberry pollen under 20℃.Compared with 20℃,lower temperature could significantly extend the living time of bayberry pollen.After storage for 100 days at 4℃,-20℃ and-80℃,the pollen germination rate was 12%,37.5% and 48%,respectively.
- 【文献出处】 浙江农业学报 ,Acta Agriculturae Zhejiangensis , 编辑部邮箱 ,2013年05期
- 【分类号】S667.6
- 【被引频次】8
- 【下载频次】260