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杨梅花粉活力检测及储藏方法研究

Vitality test and storage method for bayberry pollen

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【作者】 戚行江任海英郑锡良邱立军梁森苗张真真

【Author】 QI Xing-jiang;REN Hai-ying;ZHENG Xi-liang;QIU Li-jun;LIANG Sen-miao;ZHANG Zhen-zhen;Institute of Horticulture,Zhejiang Academy of Agricultural Sciences;Dinghai District Bureau of Agriculture and Forestry;

【机构】 浙江省农业科学院园艺研究所浙江省舟山市定海区农林局

【摘要】 对杨梅(Myrica rubra Sieb.&Zucc.)花粉活力检测和储藏方法进行了研究。筛选出最佳的杨梅花粉活力离体萌发检测法:用毛笔轻刷花粉至固体培养基(15%蔗糖,0.01%硼酸,1%琼脂)上,22℃黑暗培养10h,显微观测花粉萌发率。20℃储藏时,经硅胶干燥处理能延长杨梅花粉活力保存时间。与20℃相比,低温保存能明显延长杨梅花粉活力,在4,-20和-80℃保存100 d后,花粉萌发率分别是12%,37.5%和48%。

【Abstract】 In order to find the best storage method for pollen of bayberry(Myrica rubra Sieb.& Zucc.),the study on the optimal examination method for vitality of bayberry pollen was carried out.The results showed that bayberry pollen germinated best on the solid medium containing 15% sucrose,0.01% boric acid and 1% agar in darkness at 22℃ for 10 h.The silica gel drying could extend the living time of bayberry pollen under 20℃.Compared with 20℃,lower temperature could significantly extend the living time of bayberry pollen.After storage for 100 days at 4℃,-20℃ and-80℃,the pollen germination rate was 12%,37.5% and 48%,respectively.

【关键词】 杨梅花粉活力检测贮藏方法
【Key words】 bayberrypollenvitality teststorage method
【基金】 国家公益性行业(农业)科研专项经费项目(201203089);浙江省农业新品种选育重大科技专项(2012C129043)
  • 【文献出处】 浙江农业学报 ,Acta Agriculturae Zhejiangensis , 编辑部邮箱 ,2013年05期
  • 【分类号】S667.6
  • 【被引频次】8
  • 【下载频次】260
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