【摘要】 目的 研究穿心莲内酯（Andro）对内毒素（LPS）诱导的急性肺损伤（ALI）大鼠的保护作用及机制。方法 32只健康成年大鼠随机分成四组。生理盐水对照组、穿心莲内酯对照组、内毒素组和穿心莲内酯治疗组。对内毒素组和穿心莲内酯治疗组大鼠通过鼠尾静脉注射LPS（5 mg/kg）。给予LPS后3 h、6 h及12 h,对穿心莲内酯对照组和穿心莲内酯治疗组大鼠腹腔注射穿心莲内酯（10 mg/kg）。注射LPS后24 h收集各组支气管肺泡灌洗液（BALF）及肺组织。检测BALF中肿瘤坏死因子-α（TNF-α）、白细胞介素-1β（IL-1β）及细胞间黏附因子-1（ICAM-1）的浓度;检测肺组织中的丙二醛（MDA）、核转录因子-κB（NF-κB）浓度及髓过氧化物酶（MPO）活性,同时进行动脉血气分析。结果 与LPS组比较,穿心莲内酯治疗组TNF-α降低（P<0.05）,而IL-1β和ICAM-1明显降低（P<0.01）。穿心莲内酯治疗组与LPS组比较,MPO活性降低（P<0.05）,而MDA和NF-κB明显降低（P<0.01）。穿心莲内酯治疗组与LPS组比较,PaO₂明显升高（P<0.01）,pH值升高（P<0.05）,PaCO2降低（P<0.05）。结论 穿心莲内酯通过抑制NF-κB激活对LPS诱导的ALI具有保护作用。更多还原

【Abstract】 Objective This study was conducted in order to elucidate the protective effect and mechanism of andrographolide in lipopolysaccaride（LPS）-induced acute lung injury（ALI） of rats.Methods A total of 32 pathogen-free adult Sprague-Dawley rats were divided into 4 groups.LPS（5mg/kg） was administered intravenously via the tail vein.Andro（10 mg/kg） was injected intraperitoneally 3,6,and 12 hours after LPS injection.Broncho-alveolar lavage fluid（BALF） and lung tissues were obtained to evaluate the All at 24 hours after LPS injection.The concentration of tumor necrosis factor a（TNF-α）,interleukin 1β（IL-1β） and intercellular adhesion molecule-1（ICAM-1） were measured in BALF.Nuclear factor kB（NF-κB）,malondialdehyde（MDA） and myeloperoxidase（MPO） were measured using lung tissues.Simultaneously,artery blood gas levels were detected in each group.Results The concentration of TNF-α in BALF was lower（P < 0.05） and the concentration of IL-1β and ICAM-1 were significantly lower（P < 0.01） in Andro treatment group compared with LPS group.The concentration of MPO activity in Andro treatment group was lower than that of LPS group（P <0.05）.The concentration of MDA and NF-κB in Andro treatment group were significantly lower（P < 0.05） than that of LPS group（P < 0.01）.PaO₂ in Andro treatment group was significantly increased（P < 0.01） and pH was increased（P < 0.05） than that of LPS group.PaCO₂ in Andro treatment group were lower than that of LPS group（P < 0.05）.Conclusion Andrographolide protects against LPS-induced ALI of rats through the inhibition of NF-κB activation.更多还原