【摘要】 为确定一株ATP高产毛霉菌ZGB1在分类学上的地位,通过形态观察、生理生化特性分析、18S与ITS rDNA序列比对,对该菌进行鉴定.ZGB1形态上与放射毛霉属相似.最适生长温度为28℃;在PDA培养基中生长对数期为6～36h;最适pH为6.5;最适碳氮源分别为葡萄糖和酵母浸膏.18SrDNA序列(Accession No.JN604987)与雅致放射毛霉同源性为99%,ITS rDNA序列(Accession No.JN887460)与雅致放射毛霉两个亚种(Actinomucor elegans var.strainATCC52360及Actinomucor elegans strain CBS111556)同源性皆为100%.鉴定该菌为雅致放射毛霉的突变株,命名为Actinomucor elegans strain ZGB1.该菌能以腺嘌呤高效合成ATP,当腺嘌呤质量浓度为3g/L时,ATP产量可达9.84g/L,摩尔转化率为80.46%.更多还原

【Abstract】 To determine the status of a MucorZGB1 in taxonomy,which can efficiently synthesise ATP,it was identified based on morphological observation,physiological and biochemical property analysis,18S and ITS rDNA sequence analysis.The optimum temperature for growth was 28 ℃.The range of its log growth phase was 6~36 h.The adequate pH was 6.5.The optimum carbon was glucose.Its optimum nitrogen source was yeast extract.The homology of 18S rDNA sequences(Accession No.JN604987)of Mucor ZGB1 with Actinomucor elegans was 99%.The homology of ITS rDNA sequences(Accession No.JN887460)of Mucor ZGB1 with two subunits of Actinomucor elegans(Actinomucor elegans var.strain ATCC52360 and Actinomucor elegans strain CBS111556)was 100%.So ZGB1 was identified as one mutant strain of Actinomucor elegans and named Actinomucor elegans strain ZGB1.9.84 g/L ATP was accumulated from 3 g/L adenine and the molar conversion ratio was 80.46%.更多还原