【摘要】 从污水处理厂收集的土壤中分离到一株能降解甲醛的菌株,经过形态学分析、生理生化鉴定和16SrDNA序列比对分析,鉴定该菌为Methylobacteriumsp.XJLW.经驯化后,该菌株对甲醛的耐受由0.1g/L提高至1.2g/L.通过单因素实验得到该菌株降解甲醛的优化条件为:酵母膏1g/L,KH2PO40.7g/L,K2HPO40.8g/L,MgSO40.5g/L,温度30℃,pH 7.0.在优化后的条件下,培养52h后,该菌株对1.2g/L甲醛的降解率为31%.此外,该菌株的休止细胞8h后对2,15,30,45,60g/L的甲醛降解率分别为100%,96.8%,84.0%,26.5%,22.5%,具备较高的降解能力.更多还原

【Abstract】 A formaldehyde-degrading bacterium was isolated from soil of a wastewater treatment plant.Based on the physiological,biochemical test and 16S rDNA sequence analysis results the bacterium was identified as Methylobacterium sp.XJLW.After a period of domestication,the strain tolerance of formaldehyde concentration was up to 1.2 g/L.After single-factor tests,the optimal conditions were determined as follows: yeast extract 1 g/L,KH2PO40.7 g/L,K2HPO4 0.8 g/L,MgSO40.5 g/L,temperature 30 ℃,pH 7.0.Under the best conditions,31% of 1.2 g/L formaldehyde was degraded in 52 h.In addition,the formaldehyde degradation activity of the resting cells of Methylobacterium sp.XJLW was strongly high.When the initial concentration of formaldehyde was 2,15,30,45,60 g / L,the formaldehyde degradation rates were 100%,96.8%,84%,26.5%,22.5% in 8 h,respectively.更多还原