Objectives To prepare a recombinant Schistosoma japonicumribonuclease T2 protein(rSj RNase T2)and investigate its biological function. Methods A pair of primers with restriction sites was designed in accordance with the DNA sequence of the open reading frame encoding Sj RNase T2.The DNA fragment encoding the mature peptide of Sj RNase T2 was amplified from the plasmid DNA of CP1412/pEU-GST.This DNA fragment was subcloned into the expression plasmid pET28a(+)to construct the recombinant expression plasmid Sj...
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