Objective To study the symbiosis of Mycoplasma hominis and Trichomonas vaginalis clinical isolates and observe their symbiotic effect on AP33 sequences. Methods Primers specific to M.hominis and the AP33 gene were used for PCR amplification of 41 T.vaginalis clinical isolates.Amplification products were analyzed using 1.5%agarose gel electrophoresis.Amplification products of the AP33 gene were sequenced,and a phylogenetic tree was constructed by comparing sequencing results with the known sequences in GenBa...