【摘要】 目的观察不同种株利什曼原虫抗原的异质性。方法培养杜氏利什曼原虫、热带利什曼原虫及婴儿利什曼原虫前鞭毛体及体外转化无鞭毛体,提取总蛋白,经SDS-PAGE电泳后,分别以抗杜氏利什曼原虫及热带利什曼原虫前鞭毛体血清进行Western blot,分析抗原的多样性。结果经SDS-PAGE电泳和考马斯亮蓝染色,杜氏利什曼原虫、热带利什曼原虫及婴儿利什曼原虫前鞭毛体及体外转化无鞭毛体总蛋白呈现出分子质量单位为12～150ku的相似蛋白带型;Western blot显示,抗杜氏利什曼原虫前鞭毛体多克隆血清识别的抗原组分与抗热带利什曼原虫前鞭毛体多克隆血清识别的抗原相似;3种利什曼原虫前鞭毛体和无鞭毛体存在48、65及35ku共同抗原,杜氏利什原虫和婴儿利什曼原虫的无鞭毛体存在38ku期特异抗原,热带利什曼原虫的前鞭毛体和无鞭毛体存在45、24ku种特异抗原。结论杜氏利什曼原虫、热带利什曼原虫及婴儿利什曼原虫间存在抗原多样性,为认识利什曼病感染免疫提供了新的视角。更多还原

【Abstract】 Objective To investigate the antigenic diversity of different Leishmania spp.Methods Leishmania donovani,Leishmania tropica,and Leishmania infantum promastigotes were cultured and transformed into amastigotes in vitro.Total protein from the promastigotes and amastigotes was separated with SDS-PAGE,and antigenic diversity was identified using polyclonal serum against L.donovani promastigotes and L.tropica promastigotes in Western blotting.Results Following SDS-PAGE electrophoresis and Coomassie brilliant blue staining,the total protein of different Leishmania spp.promastigotes and amastigotes resulted in similar visible bands,and proteins ranged in molecular weight from 12 ku to 150 ku.Western blotting showed that similar antigens were identified by polyclonal sera against L.donovani and L.tropica.Promastigotes and amastigotes of the 3 different Leishmania spp.had similar antigens of 48 ku,65 ku,and 35 ku.A 38-ku stage-specific antigen was present in L.donovani amastigotes and L.infantum amastigotes,and 45-ku and 24-ku species-specific antigens were present in L.tropica promastigotes and amastigotes.Conclusion Antigenic diversity is present in different Leishmania spp.This finding provides new insight into understanding immunity from leishmaniasis.更多还原