Objectives To sequence and compare the mtDNA-COⅠ variance of the 5 members of Anopheles maculatus s.l.(An.pseudowillmori,An.maculatus s.s,An.willmori,An.dravidicus,and An.sawadwongporni) and analyze their phylogenetic relationship.Methods An.maculates s.l.was identified by morphology and multiple PCR with variance in the ITS2 gene sequence,and then mtDNA-COⅠ was amplified and sequenced.Chromas and Bioedit software were used to compare the gene sequence and Mega 3.0 was used to construct a phylogenetic tree....