【摘要】 目的探讨正常成人胰岛移植治疗糖尿病的作用。方法 2012年1月至2013年1月健康成人胰腺供体共13例。获取胰腺后经胶原酶消化并进行胰岛提取纯化,将纯化后的胰岛移植到糖尿病裸鼠肾包膜下为实验组,生理盐水代替胰岛移植为对照组,各13只。术后通过检测糖尿病裸鼠的血糖变化和组织学检查来判定胰岛移植后的存活情况和纠正血糖的效果。结果成人胰腺消化后胰岛产量为纯化前(3608±403)IEQ/g,纯化后(2820±318)IEQ/g,纯度为85%。实验组84.6%(11/13)糖尿病裸鼠在移植术后第1天,血糖降至正常,对照组血糖无明显变化。两组糖尿病裸鼠存活时间:实验组存活时间35～57d,中位存活时间46d。对照组存活时间5～12d,中位存活时间7d。两组比较差异具有统计学意义,Log-rank(Mantel-Cox)值为8.74,P值为0.0012。结论成人胰腺消化后,可得到高纯度活力正常的胰岛,并能纠正糖尿病裸鼠的高血糖。正常成人胰岛移植可能成为临床糖尿病患者的一种有效治疗方法。更多还原

【Abstract】 Objective To investigate the efficiency of adult pancreatic islets in the treatment of diabetic patients.Methods Thirteen adult donor panceata were used for this study.After collagenase digestion,the islets were isolated and purified followed by transplantation into diabetes SPF nude mice.Control group were transplanted by normal saline solution.Islet graft survival was monitored by the detection of glucose changes in diabetic mice and histological examination.Results Islet yield was(3608±403) IEQ /g in this study,while that was(2820±318)IEQ/g after purification with a purity of 85%.The blood glucose of 84.6% diabetic mice decreased to normal in the first day post-transplantation.Normal blood glucose level maintained for a long time.There was significant difference in the Kaplan-Meier analysis for the islet survival(P = 0.0012).Conclusion The adult donor pancreas is an ideal source for islet transplantation due to high yield and good function with a high purity and viability to correct the diabetic nude mice blood glucose level.更多还原