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响应面分析法优化紫芝多糖的脱色工艺

Optimization of Decolorization Technology of Ganoderma sinense Polysaccharides by Response Surface Methodology

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【作者】 杨悦文胡晓杨义芳罗永明廖建维

【Author】 YANG Yuewen1,HU Xiao2,YANG Yifang2*,LUO Yongming1,LIAO Jianwei3(1.Jiangxi University of Traditional Chinese Medicine,Nanchang 330004;2.State Key Lab.of New Drug and Pharmaceutical Process,Shanghai Institute of Pharmaceutical Industry,China State Institute of Pharmaceutical Industry,Shanghai 200040; 3.Hisun Pharmaceutial Co.,Ltd.,Taizhou 318000)

【机构】 江西中医学院中国医药工业研究总院上海医药工业研究院创新药物与制药工艺国家重点实验室海正药业股份有限公司

【摘要】 根据Box-Behnken实验设计原理,采用3因素3水平的响应面分析法优化紫芝多糖树脂脱色工艺条件,依据回归分析确定各工艺条件的影响因子,以脱色率为响应值作响应面和等高线。结果表明曲面回归模型能较好地预测各因素与响应值之间的关系,优化得到紫芝多糖脱色最佳工艺条件为:脱色时间2.5 h,树脂用量0.75 g/10 ml,脱色温度28℃。在上述条件下,紫芝多糖实际脱色率为87.8%。

【Abstract】 Based on the Box-Behnken design principles,the method of response surface methodology with 3 factors and 3 levels was adopted on optimizing the resin decolorization technology of Ganoderma sinense polysaccharides.The factors influencing the technological parameters were determined by means of regression analysis.Response surface and contour were finally graphed with the decolorization rate as the response value.The optimal decolorization conditions were as follows: decolorization time of 2.5 h,0.75 g/10 ml resin and temprature of 28 ℃.Under such conditions,the actual decolorization rate of Ganoderma sinense polysaccharides was 87.8%.

【基金】 国家“重大新药创制”科技重大专项(2009ZX09301-007);上海市中药现代化专项资助项目(06DZ19706);上海市自然科学基金资助项目(05ZR14112)
  • 【文献出处】 中国医药工业杂志 ,Chinese Journal of Pharmaceuticals , 编辑部邮箱 ,2013年04期
  • 【分类号】TQ461
  • 【被引频次】6
  • 【下载频次】249
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