【摘要】 目的:对灯盏花群体种质资源进行分析,为灯盏花的品种选育提供依据。方法:用筛选出来的11对引物对采自云南泸西、昆明、大理、丘北、石林的6份灯盏花种质资源进行AFLP遗传多样性分析。结果:①11个引物在6份种质资源中共产生636条DNA片段,其中604条带为多态性条带,约占82.40%。②在相似系数为0.706时,6份不同地区的灯盏花可聚为3类,第1类包括千山1号和千山2号大部分样品及大理、石林、昆明3个地区的野生居群;第2类为丘北的野生居群;第3类主要为部分千山2号及其他地区样品。千山1号和千山2号灯盏花平均遗传相似系数大,遗传相似系数变幅小,品系内遗传分化较小,遗传性状较稳定;③对6份灯盏花样品的分子系统聚类分析表明:地理来源相同的部分有相对聚合现象或少部分品系出现交差聚类,与其亲缘关系较近有关;丘北居群自行一类,与其特异的遗传基础差异较大有关。结论:AFLP对灯盏花的遗传多样性分析具有可靠性;系统选育对灯盏花的育种具有可行性。更多还原

【Abstract】 Objective: To analyze the genetic diversity and breeding strains of the E.breviscapus germplasms,in order to provide theoretical information for Erigeron breviscapus breeding.Method: The genetic diversity and genetic structure were assayed to six germplasm resource of E.breviscapus which collected from Yunnna with 11 pairs primers and AFLP molecular marker.Result: Six hundred and four amplification bands among 636 DNA bands were from six accession of E.breviscapus,which are about 82.40% of total bands.The six germplasms could be divided into three group at the 0.706 similarity coefficient level.The first category include QS-1,QS-2 and Dali,Shilin,Kunming population.The second category included wild population of Qiubei.The third category included several sample from different district.The mean genetic similarity coefficient of QS-1 and QS-2 was bigger,genetic similarity coefficient range was smaller,hereditary character was more stable.Molecular system clustering analysis showed that the geographical origin of the same part had relative polymerization phenomenon and its genetic relationship was close.Qiubei was a single group possibly relating to the specific genetic basis.Conclusion: The analysis of genetic diversity of E.breviscapus by AFLP marker is reliable.The systematic E.breviscapus breeding is feasible.更多还原