【摘要】 通过对新疆紫草Arnebia euchroma红白2种色系悬浮细胞的表型、生长曲线、次生代谢产物积累研究,用数学建模模拟新疆悬浮细胞的增殖和紫草素类化合物积累情况,研究新疆紫草悬浮细胞生长和次生代谢生产的动力学参数,发现红色高产系为针对紫草素类次生代谢产物生产的优良细胞株系,白色低产系可能为突变株系。用Logistic模型对生长函数进行拟合并求导数,得到新疆紫草悬浮细胞的生长速率及生长加速度变化曲线,确定了新疆紫草悬浮细胞继代培养最佳时期为继代到新鲜培养基中的第15天,给诱导处理的最佳时期为继代到新鲜培养基中后第13～14天。并且通过比较新疆紫草红色系细胞增长速率的变化趋势与紫草素类化合物积累曲线发现,新疆紫草悬浮7细胞红色系生物量快速增长时不利于紫草素的合成与积累。更多还原

【Abstract】 Via studying the phenotype,growth curve and secondary metabolites of two kinds of suspension culture cell of Arnebia euchroma,the kinetics parameters of growth and accumulation of shikonin compounds in cell suspension culture of A.euchroma was obtained through simulating and modeling.This Study found that the red high-yielding one was a fine cell line for producing shikonin compounds,and the white low-yielding one may be a mutant.The first-order and second-order derivative of the fitting function were obtained by fitting the Logistic model of growth curve to get the growth rate and growth acceleration curve of the suspended cells.It is found that the best period to subculture was the 15th day cultured in fresh medium,and the best period of the induction process was the 13th-14th day.When compared the growth rate of the red line and the shikonin compounds accumulation curve,it is found that the rapid growth of the biomass of cells was not conducive to the synthesis and accumulation of shikonin compounds.更多还原