【摘要】 目的:建立藏药"蒂达"不同药材品种的薄层色谱鉴别方法。方法:采用硅胶G板、三氯甲烷-甲醇(40∶1)展开,分离鉴别"蒂达"不同药材品种中的齐墩果酸成分。结果:对70个来源于龙胆科獐牙菜属、花锚属、扁蕾属、肋柱花属及虎耳草科虎耳草属的"蒂达"各品种药材中齐墩果酸的薄层色谱鉴别表明,除虎耳草外,其他来源品种中均检出有齐墩果酸,同种的各样品间薄层图谱特征一致,但同属不同种间及同科不同属间薄层图谱特征存在一定差异。结论:齐墩果酸的薄层鉴别方法可用于"藏茵陈"药材部分种的鉴别,可以有效鉴别来源于虎耳草科与其他种的"蒂达"样品。更多还原

【Abstract】 Objective: To establish a method of TLC identification for Dida commonly used in Tibetan medicine from different species.Method: With silica gel G as the stationary phase,and chloroform-methanol(40∶1) as mobile phase,oleanolic acid from different species of Dida was separated and identified.Result: Oleanolic acid was detected in 70 kinds of Dida derived from the Gentianaceae Swertia,Halenia,Gentianopsis,Lomatogonium,and Saxifragaceae saxifrage,except for the saxifrage,there are some differences among different genera or subjection.Conclusion: This TLC method can be used for identification of oleanolic acid in Dida from different species except saxifrage.更多还原