【摘要】 目的:建立同时测定银黄薄膜衣片中绿原酸和黄芩苷含量的方法。方法:采用HPLC等度洗脱法,C18色谱柱(4.6 mm×250 mm,5μm),流动相甲醇-水-磷酸(45∶55∶0.2),流速1.0 mL.min-1,柱温30℃,检测波长328 nm,进样量20μL。结果:绿原酸和黄芩苷在2.200～44.00 mg.L-1和24.20～242.0 mg.L-1峰面积与进样量呈良好的线性关系,平均回收率分别为99.63%(RSD 1.8%,n=9)和99.72%(RSD 1.9%,n=9)。结论:该方法简便、准确,可同时测定银黄薄膜衣片中绿原酸和黄芩苷的含量。更多还原

【Abstract】 Objective: To establish an HPLC method for determination of chlorogenic acid and baicalin in Yinhuang film-coated tablets.Method: HPLC isocratic elution was adopted,with the C18 column(4.6 mm × 250 mm,5 μm) as the chromatographic column,methanol-water-phosphoric acid(45∶55∶0.2) as the mobile phase.The flow rate was set at 1.0 mL·min-1,and column temperature was set at 30 ℃.The detection wavelength was 328 nm,and the sample size was 20 μL.Result: At concentrations ranging between 2.200-44.00,24.20-242.0 mg·L-1,peak area of chlorogenic acid and baicalin showed good linear relationship,the average recoveries were 99.63%(RSD 1.8%,n=9) and 99.72%(RSD 1.9%,n=9),respectively.Conclusion: The method is simple that it can accurately measure the content of chlorogenic acid and baicalin in Yinhuang film-coated tablets.更多还原