【摘要】 目的:探讨在大鼠肝外淤胆模型中大胆管和小胆管原纤毛形态的变化。方法:将15只Wistar大鼠随机平分为未处理组(ratsctr)、胆管结扎7d组(ratsL7)和胆管结扎10d组(ratsL10)。用胆总管结扎法制备大鼠肝外淤胆模型。利用扫描电镜技术,比较肝内大、小胆管上皮细胞原纤毛在长度和数量上的异同。结果:ratsctr组大胆管上皮细胞原纤毛平均长度为(7.76±1.14)μm,数量为每视野(10000×)(12±3)根。胆汁淤积后,大胆管上皮细胞原纤毛长度变短:ratsL7为(2.92±1.39)μm,P<0.05;ratsL10缩短为(2.34±0.37)μm,P<0.05,数量增多:ratsL7为(87±12)根,P<0.05;ratsL10为(197±46)根,P<0.05。正常大鼠小胆管上皮细胞原纤毛的平均长度为(5.15±0.89)μm,数量为每视野(4±1)根。胆汁淤积后,小胆管上皮细胞原纤毛长度变短:ratsL7为(2.52±0.96)μm,P<0.05;ratsL10为(2.31±0.53)μm,P<0.05,数量增多:ratsL7为(16±2)根,P<0.05;ratsL10为(15±3)根,P<0.05。结论:结扎胆总管导致肝脏淤胆时,胆管上皮细胞原纤毛数量增多,长度变短。更多还原

【Abstract】 Objective To study the morphology of cholangiocyte cilia in large bile ducts(LBDs) and small bile ducts(SBDs) of rats with extra-hepatic cholestasis induced by bile duct ligation(BDL).Methods Fifteen rats were divided randomly into three groups(5 in each).Rats in these groups were subjected to 7-day BDL(ratsL7),10-day BDL(ratsL10) and no special treatment(ratsctr).We characterized the primary cilia of cholangiocytes in these rats using scanning electron microscope.Results In the large bile ducts of ratsctr,the average length and number of primary cilia were(7.76 ± 1.14) μm and(12 ± 3) /field,respectively.Compared to the control values,the primary cilia in LBDs were decreased in length(ratsL7 2.92 ± 1.39,P < 0.05 and ratsL10 2.34 ± 0.37,P<0.05) and increased in numbers(ratsL7 87 ± 12 /field,P<0.05 and ratsL10 197 ± 46 /field,P<0.05) as a result of BDL.In the SBDs of ratsctr,the average length and number of primary cilia were 5.15 ± 0.89 μm and 4 ± 1 /field,respectively.Compared to the control values,the primary cilia in SBDs were decreased in length(ratsL7 2.52 ± 0.96,P<0.05 and ratsL10 2.31 ± 0.53,P<0.05) and increased in numbers(ratsL7 16 ± 2 /field,P<0.05 and ratsL10 15 ± 3 /field,P<0.05) as a result of BDL.Conclusion After rat BDL,cholangiocyte cilia were increased in number and decreased in length.更多还原