【摘要】 目的:探讨miRNA-375的外源性表达对人胰腺癌细胞Panc-1增殖和凋亡的影响。方法:将miRNA-375表达质粒或对照质粒转染Panc-1细胞,qRT-PCR方法检测miRNA-375在转染细胞中的表达水平,细胞计数法、流式细胞术分别检测外源性miRNA-375表达后Panc-1细胞增殖、凋亡和周期的变化情况。结果:miRNA-375表达质粒组与对照组比较,其miRNA-375表达诱导性升高,细胞增殖受到抑制,细胞凋亡率增加,细胞周期阻滞于G0～G1期。结论:在胰腺癌细胞Panc-1中增加miRNA-375的表达,可抑制细胞增殖,促进细胞凋亡和细胞周期阻滞,miRNA-375在胰腺癌细胞Panc-1中可能发挥潜在的抑癌基因作用。更多还原

【Abstract】 Objective: This study aimed to investigate the effect of the ectogenesis expression of miRNA-375 on cell proliferation and apoptosis in the human pancreatic cancer cell line Panc-1.Methods: After transfection of the miRNA-375 vector,the expression levels of miRNA-375 in the Panc-1 cells were determined by quantitative real-time polymerase chain reaction.Cell counting was performed to evaluate the effects of miRNA-375 expression on cell proliferation.Flow cytometry was used to analyze apoptosis and the cell cycle.Results: The expression levels of miRNA-375 were highly induced after miRNA-375 transfection.miRNA-375 restoration also inhibited cell proliferation,promoted cell apoptosis,and induced cell cycle arrest at G0-G1.Conclusion: The abnormal expression of miRNA-375 in the pancreatic cancer cell line Panc-1 inhibits the canceration of cells by interfering with cellular biological behaviors,such as proliferation and apoptosis.miRNA-375 may act as a potential tumor suppressor in the pancreatic cancer cell Panc-1.更多还原