目的:探讨从五步蛇毒中分离纯化凝血酶样酶的有效方法。方法:用Superdex 75、Superdex 30凝胶过滤色谱和DEAE-Sepharose Fast Flow阴离子交换色谱技术层析,SDS-PAGE分析纯度。结果:从五步蛇毒中分离纯化得到一个具有凝血活性的组分。SDS-PAGE显示为单一条带,相对分子质量约25 kDa。结论:用Superdex75、Superdex30凝胶过滤色谱和DEAE-Sepharose Fast Flow阴离子交换色谱技术可以很好分离纯化五步蛇毒中凝血酶样酶。
【英文摘要】
OBJECTIVE To explore a method of purification of thrombin-like enzyme from Agkistrodon acutus venom (AAV).METHODS Thrombin-like enzyme(TLE)from AAV was isolated and purified by Gel filtration chromatography on Superdex75,Superdex30 and DEAE-Sepharose Fast Flow ion-exchange,SDS-polyacrylmide gel electrophoresis(SDS-PAGE) was used to analyze its purity.RESULTS One of thrombin-like enzyme fromAgkistrodon acutus venom was homogeneous as a single band showed on SDS-PAGE with molecular weight about 25 kDa as esti...
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