【摘要】 目的:考察普萘洛尔对盐酸二甲双胍肾排泄的影响。方法:20只健康Wistar雄性大鼠随机分为2组,一组尾静脉注射盐酸二甲双胍和普萘洛尔,另一组注射盐酸二甲双胍,于给药后收集各时间段(0～2,2～4,4～6,6～8,8～10,10～12,12～24 h)的尿液样品。用高效液相色谱法测定二甲双胍尿药浓度,用DAS 2.0软件计算二甲双胍的药动学参数后对数据进行分析。结果:联合用药组的药动学参数t1/2,Ke[(2.8±0.4)h,(0.25±0.58)h-1]较二甲双胍组[(2.11±0.30)h,(0.34±0.45)h-1]存在统计学差异(P<0.05),但总尿排量较二甲双胍组无显著性差异(P>0.05)。结论:普萘洛尔对健康Wistar雄鼠盐酸二甲双胍的肾排泄速率有显著影响,可能与其竞争性抑制了肾脏rOCT2有关。更多还原

【Abstract】 OBJECTIVE To study the effects of co-administration of propranolol on the renal excretion of metformin hydrochloride in rats.METHODS 20 young male rats were randomly divided into 2 equal groups.After the rats of the co-administration group treated with both propranolol and metformin hydrochloride,metformin hydrochloride group were treated only with metformin hydrochloride via tail vein injection,the urine samples were collected in 0-2,2-4,4-6,6-8,8-10,10-12 and 12-24 h.The concentrations of metformin hydrochloride in urine were determined by high performance liquid chromatography.According to the concentration of urine,the software of DAS 2.0 was used to calculate the pharmacokinetic parameters.RESULTS The significantly changed pharmacokinetic parameters of the metformin group and the testing group(P<0.05) in rats:t1/2 were(2.8±0.4) and(2.11±0.30) h,respectively;Ke were(0.25±0.58) and(0.34±0.45) h-1 respectively;while the total urine discharge of metformin was not significantly changed(P>0.05).CONCLUSION Propranolol had significant effect on the renal excretion process of metformin hydrochloride in rats;the possible reason was that propranolol may competitively inhibit renal rOCT2 in rats.更多还原