【摘要】 目的建立小菜蛾抗菌肽CA的高效液相色谱测定方法。方法色谱柱为Agilent Alltima C18(4.6 mm×250 mm,5μm),流动相为甲醇-乙腈-0.05%三氟乙酸溶液(2.5∶2.5∶95),检测波长为275 nm,柱温为25℃,流速为1.0 mL·min-1。结果小菜蛾抗菌肽CA的线性范围为0.08～0.800μg(r=0.999 4,n=6),平均回收率分别为99.69%,RSD为1.20%(n=6)。结论本法快速、简便、准确,重现性好,可用于小菜蛾抗菌肽CA的定量分析和质量控制。更多还原

【Abstract】 OBJECTIVE To establish a method for determination of cecropin from Plutella xylostella(pxCECA1) by RP-HPLC.METHODS An Agilent Alltima C18 column(4.6 mm×250 mm,5 μm) was adopted,and the mobile phase was composed of methanol-acetonitrile-0.05% TFA(2.5∶2.5∶95) at a flow rate of 1.0 mL·min-1.The detection wavelength was set at 275 nm and the column temperature was 25 ℃.RESULTS The linear range of the calibration curve for cecropin was 0.08-0.800 μg(r=0.999 4,n=6).The average recovery was 99.69%,and RSD was 1.20%(n=6),respectively.CONCLUSION The method is rapid,simple,and accurate with good reproducibility.It can be used for quantitative analysis and quality control of cecropin from Plutella xylostella.更多还原