【摘要】 目的研究桔梗皂苷D对人结肠癌SW620细胞增殖的影响及作用机制。方法采用人结肠癌细胞株SW620细胞,分别以四甲基偶氮唑蓝法观察桔梗皂苷D对细胞增殖的抑制作用,以流式细胞术检测桔梗皂苷D对细胞周期分布和凋亡的影响,蛋白质免疫印迹法检测桔梗皂苷D对细胞周期蛋白和凋亡蛋白表达的影响。结果与空白对照组比较,桔梗皂苷D呈剂量依赖地抑制人结肠癌SW620细胞的生长;15、20μmol·L-1桔梗皂苷D作用细胞24h后,显著增加G0/G1期细胞比例,分别为(72.83±5.26)%和(76.82±5.83)%(P<0.05,空白对照组(56.78±4.92)%,周期蛋白cyclinD1、c-myc、CDK6表达明显下调;10、15、20μmol·L-1桔梗皂苷D作用细胞48h后,明显增加细胞凋亡率,分别为(19.5±5.1)%、(35.8±5.3)%和(43.8±4.0)%(P<0.05,空白对照组(8.93±5.72)%,相关蛋白pro-caspase3、PARP表达下降。结论桔梗皂苷D通过调控周期蛋白cyclinD1、c-myc、CDK6的表达,将细胞阻滞于G1期,进而诱导细胞凋亡,抑制人结肠癌细胞SW620的增殖。更多还原

【Abstract】 OBJECTIVE To explore the inhibitory effect and its mechanism of platycodin D(PD)on human colonic cancer SW620 cell proliferation.METHODS The inhibitory effect of PD on SW620 cell proliferation was analyzed by MTT assay,while the effect of PD on cell cycle distribution and apoptosis were evaluated by flow cytometry.The effect of PD on expression of cyclin and apoptosis associated proteins were detected by Western blot.RESULTS The cell proliferation of human colonic cancer SW620 cell was inhibited by PD in a dose-dependent manner.After cells were treated with PD(15,20 μmol·L-1) for 24 h,the proportions of cells in G0/G1 phase were(72.83±5.26)% and(76.82±5.83)% respectively,while the control group cells was(56.78±4.92)%,which suggested PD could block SW620 in the G1 phase compared with the control group cells.Furthermore,the expressions of cyclinD1,c-myc and CDK6 were reduced obviously.Compared with the control group cells,the apoptotic rates were increased,[(19.5±5.1)%,(35.8±5.3)% and(43.8±4.0)% respectively] after cells were treated by PD(10,15,20 μmol·L-1) for 48 h.The expression of procaspase 3 and PARP with proenzyme form were reduced.CONCLUSION PD could inhibit the growth of colon cancer cell by blocking SW620 in the G1 phase through regulating the expression of cyclinD1,c-myc and CDK6 and thus inducing the apoptosis by cell cycle arrest.更多还原