【摘要】 目的 观察茶多酚对脂多糖(LPS)诱导下人牙周膜成纤维细胞(HPDLFs)增殖的影响。方法 采用组织块培养法培养原代HPDLFs并传代、免疫组化检测,取第5代细胞用于实验。将LPS及茶多酚分组,采用四甲基偶氮唑盐(MTT)法分别于24、48、72 h检测HPDLFs增殖活力。结果 免疫组化染色结果显示,所培养细胞的波形丝蛋白染色呈阳性表现,阳性部位位于胞质,角蛋白染色呈阴性表现。各浓度LPS组在24、48、72 h的光密度(OD)值(4 mg/L LPS组:0.323±O.007、0.345±0.010、0.437±0.007;20 mg/L LPS组:0.270±0.004、0.283±0.009、0.367±0.007;100 mg/L LPS组:0.175±0.006、0.187±0.006、0.248±0.005:500 mg/L LPS组:0.088±0。004、0.091±0.006、0.153±0.009)与同时段空白对照组(0.306±0.008、0.333±0.007、0.399±0.007)组间比较差异均有统计学意义(均P<0.05)。100 mg/L LPS组和各浓度茶多酚组在24、48、72 h的OD值(100 mg/L LPS组:0.216±0.010、0.260±0.010、0.352±0.012;100 mg/L LPS组+0.125 g/L茶多酚组:0.300±0.010、0.408±0.013、0.490±0.014;100 mg/L LPS组+0.25 g/L茶多酚组:0.333±0.012,0.416±0.010、0.532±0.012;100mg/LLPS组+0.5 g/L茶多酚组:0.390±0.012,0.485±0.011、0.642±0.013;100 mg/LLPS组+1 g/L茶多酚组:0.368±0.010、0.481±0.006、0.621±0.007)与同时段空白对照组(0.450±0.012、0.528±0.011、0.687±0.010)组间比较,差异均有统计学意义(均P<0.05);各浓度茶多酚组在24、48、72 h的OD值均低于同时段100 mg/L LPS组(均P<0.05)。结论 LPS抑制HPDLFs的增殖,茶多酚明显促进LPS诱导下HPDLFs的增殖,这提示茶多酚可应用于牙周炎的预防及治疗。更多还原

【Abstract】 Objective To observe the effects of lipopolysacharid(LPS) and tea polyphend(TP) on proliferation stimulated by LPS in human periodontal ligament cells(HPDLFs).Methods HPDLFs were primary cultured from tissue explants,and the cells of the 5th passages were used after immunohistochemistry detection.The cells were divided into LPS groups and TP groups,the proliferation of HPDLFs were examined by MTT colorimetriv assay after 24,48,72 h respectively.Results Immunohistochemical study showed that all vimentin of cell were stained positively,and the positive area were located in the cytoplasm,keratin were stained in negative.There were significant differences on the OD vaule of each concentration LPS group at 24,48,72 h(4 mg/L LPS group:0.323 ±0.007,0.345 ±0.010,0.437 ±0.007;20 mg/L LPS group:0.270 ± 0.004,0.283 ± 0.009,0.367 ± 0.007;100 mg/L LPS group:0.175 ± 0.006,0.187 ± 0.006,0.248 ± 0.005;500 mg/L LPS group:0.088 ± 0.004,0.091 ± 0.006,0.153 ± 0.009) when compared with blank control group in the same time(0.306 ± 0.008,0.333 ±0.007,0.399 ±0.007).There were significant differences on OD vaule of each concentration TP group at24,48,72 h(100 mg/L LPS group:0.216 ±0.010,0.260 ±0.010,0.352 ±0.012;100 mg/L LPS group +0.125 g/L TP group:0.300 ± 0.010,0.408 ± 0.013,0.490 ± 0.014;100 mg/L LPS group + 0.25 g/L TP group:0.333 ±0.012,0.416 ± 0.010,0.532 ± 0.012;100 mg/L LPS group + 0.5 g/L TP group:0.390 ±0.012,0.485 ±0.011,0.642 ±0.013;100 mg/L LPS group +1 g/L TP group:0.368 ±0.010,0.481 ±0.006,0.621 ±0.007) when compared with blank control group in the same time(0.450 ±0.012,0.528 ±0.011,0.687 ± 0.010).OD vaule of each concentration TP group at 24,48,72 h were lower than that of 100 mg/L LPS group in the same time(P < 0.05).Conclusions LPS inhibits the proliferation of HPDLFs.TP promotes the proliferation of LPS induced by the HPDLFs.TP can be applied to the prevention and treatment of periodontitis.更多还原