【摘要】 目的:研究人参皂苷Rh2(Gensenoside Rh2)与磷脂酰肌醇-3-激酶/丝苏氨酸蛋白激酶(PI3K/AKT)信号通路抑制剂LY294002对乳腺癌细胞转移和侵袭的影响。方法:试验分为对照(空白培养液)组、人参皂苷Rh2(80μmol/L)组、LY294002(50μmol/L)组与人参皂苷Rh2(80μmol/L)+LY294002(50μmol/L)组。人参皂苷Rh2与LY294002体外作用于人乳腺癌细胞MCF7/Adr,Western blot法检测细胞基质金属蛋白酶(MMP)2、P-糖蛋白(P-gp)、丝苏氨酸蛋白激酶(AKT)、磷酸化丝苏氨酸蛋白激酶(p-AKT)蛋白表达;黏附试验观察细胞与基底膜的黏附力,Transwell法观察细胞侵袭能力和迁移能力。结果:与对照组比较,人参皂苷Rh2组、LY294002组与人参皂苷Rh2+LY294002组p-AKT、P-gp、MMP-2蛋白表达显著减弱,细胞与基底膜的黏附力、细胞迁移能力与侵袭能力显著减弱(P<0.05);人参皂苷Rh2+LY294002组与人参皂苷Rh2组、LY294002组比较以上指标差异具有统计学意义(P<0.05)。结论:抑制PI3K/AKT信号通路可有效降低MCF7/Adr侵袭迁移能力,PI3K/AKT通路是调控乳腺癌侵袭迁移的重要信号通路之一。更多还原

【Abstract】 OBJECTIVE:To investigate the effects of gensenoside Rh2and phosphatidylnositol 3-kinase/ serine-threonine kinase(PI3K/AKT)pathway inhibitor LY294002 on invasion and migration of breast cancer cells. METHODS:Human breast cancer cell lines were divided into control group(blank culture),gensenoside Rh2(80 μmol/L)group,LY294002 group(50 μmol/L)and gensenoside Rh2(80 μmol/L)+LY294002(50 μmol/L)group. Human breast cancer cell lines MCF7/Adr were cultured with gensenoside Rh2and PI3K/Akt signaling pathway inhibitor LY294002 in vitro. The protein expressions of matrix metalloproteinases 2(MMP-2),P-glycoprotein(P-gp),AKT and p-AKT were detected by Western blot. The cell-ground substance adhesion ability was tested by cell adherence assay;the cell invasion and migration were determined by Transwell chamber model. RESULTS:Compared with control group,the protein expressions of p-AKT,P-gp and MMP-2 were decreased in gensenoside Rh2group,LY294002 group and gensenoside Rh2+LY294002 group;cell-ground substance adhesion ability and the cell invasion and migration were decreased significantly(P<0.05). There was significant difference in above index among gensenoside Rh2+LY294002 group,gensenoside Rh2group and LY294002 group(P<0.05). CONCLUSIONS:The invasion and metastasis of MCF7/Adr can be reduced significantly through suppression of the PI3K/AKT signaling pathway. PI3K/AKT signaling pathway may be an important way to modulate invasion and metastasis of breast cancer.更多还原