【摘要】 目的:研究利胆止痛胶囊的药理作用。方法:采用大鼠胆管引流法测定不同剂量利胆止痛胶囊(0.72、0.36、0.18 g/kg)对正常大鼠胆汁量的影响;以张力换能器测定不同质量浓度利胆止痛胶囊溶液(4、2、1、0.5 g/L)对豚鼠胆囊平滑肌收缩的影响;一次性灌胃异硫氰酸-1-萘酯(ANIT)复制大鼠黄疸模型,测定不同剂量利胆止痛胶囊(0.72、0.36、0.18 g/kg)对黄疸模型大鼠血清中丙氨酸氨基转移酶(ALT)、天冬氨酸氨基转移酶(AST)和总胆红素(TBIL)含量的影响;一次性皮下注射四氯化碳(0.5 ml/100 g)复制大鼠肝损伤模型,测定不同剂量利胆止痛胶囊(0.72、0.36、0.18 g/kg)对肝损伤模型大鼠血清ALT、AST、碱性磷酸酶(ALP)活性和总蛋白(TP)、白蛋白(ALB)含量与肝脏病理学的影响。结果:0.72、0.36 g/kg剂量下利胆止痛胶囊可显著增加正常大鼠的胆汁流量(P<0.01)。1.0~4.0 g/L质量浓度下利胆止痛胶囊可呈浓度相关性地增加豚鼠离体胆囊平滑肌条的张力(P<0.01或P<0.05)。0.72、0.36 g/kg剂量下利胆止痛胶囊可显著降低黄疸模型大鼠血清中TBIL含量(P<0.01或P<0.05);0.72 g/kg剂量下利胆止痛胶囊可显著降低黄疸模型大鼠血清中ALT、AST活性(P<0.01或P<0.05)。0.72 g/kg剂量下利胆止痛胶囊可显著降低肝损伤模型大鼠血清ALT、AST、ALP活性(P<0.01或P<0.05)。病理组织学观测显示,0.72、0.36 g/kg剂量下利胆止痛胶囊可改善肝损伤模型大鼠肝小叶结构破坏、肝细胞明显变性坏死和炎性细胞浸润、细胞结构凌乱等情况。结论:利胆止痛胶囊具有一定的利胆、退黄和保肝降酶作用。更多还原

【Abstract】 OBJECTIVE:To study pharmacological effects of Lidan zhitong capsule.METHODS:Effects of different doses of Lidan zhitong capsule(0.72,0.36,0.18 g/kg)on the amount of bile in normal rats were determined by the method of biliary drainage of rat.Effects of different concentrations of Lidan zhitong capsule(4,2,1,0.5 g/L)on the contraction of gallbladder smooth muscle in guinea pigs were determined by tonotransducer.The icterus model in rats was duplicated by intragastrical administration of naphthyl-1-isothiocyanate(ANIT)to observe the effects of different doses of Lidan zhitong capsule(0.72,0.36,0.18 g/kg)on alanine aminotransferase(ALT),aspartate aminotransferase(AST)and total bilirubin(TBIL)in serum.The hepatic injury model induced by disposable hypodermic injection of CCl4 in rats was produced to evaluate the effects of different doses of Lidan zhitong capsule(0.72,0.36,0.18 g/kg)on the serum levels of ALT,AST,alkaline phosphatase(ALP),total protein(TP),albumin(ALB)and hepatic histopathology.RESULTS:Lidan zhitong capsule(0.36,0.72 g/kg)could remarkably increase the amount of bile in normal rats(P<0.01).Lidan zhitong capsule(4,2,1 g/L)could elevate the tension of gallbladder muscle stripes of guinea pigs in a dose-dependent manner(P<0.01 or P<0.05).Lidan zhitong capsule(0.36 and 0.72 g/kg)could significantly decline the serum level of TBIL(P<0.01 or P<0.05);Lidan zhitong capsule(0.72 g/kg)could also lower the serum levels of ALT and AST in icterus model rats(P<0.01 or P<0.05).Lidan zhitong capsule(0.72 g/kg)could significantly decline the serum levels of ALT,AST and ALP in the hepatic injury model rats(P<0.01 or P<0.05).Pathological histology showed that Lidan zhitong capsule(0.72,0.36 g/kg) could improve the structures destruction of hepatic lobule,obvious degeneration and necrosis of hepatocytes,infiltration of inflammatory cells and the structures foul-up of cells,etc.CONCLUSIONS:Lidan zhitong capsule has obvious choleretic,jaundice-relieving and liver-protective effects.更多还原