【摘要】 目的:研究痛风泰颗粒对急性痛风性关节炎模型大鼠基质金属蛋白酶(MMP)3、脂蛋白磷脂酶(LP-PL)A2的影响。方法:实验分为空白对照(等容生理盐水)、模型对照(等容生理盐水)、双氯芬酸钠(67.5mg/kg)与痛风泰颗粒高、中、低剂量(22.68、11.34g、5.67g/kg)组。大鼠踝关节腔注入50μl尿酸钠溶液(20mg/ml)以复制急性痛风性关节炎模型。分别于给药3、7d后取关节滑膜进行组织匀浆,用ELISA法检测大鼠关节滑膜组织中MMP-3、LP-PLA2的含量。结果:给药3d后,与模型对照组比较,痛风泰颗粒高、中剂量组大鼠滑膜组织MMP-3、LP-PLA2含量显著减少(P<0.05);给药7d后,痛风泰颗粒高、中、低剂量组大鼠滑膜组织中MMP-3、LP-PLA2含量显著减少(P<0.01或P<0.05)。结论:痛风泰颗粒能减少急性痛风性关节炎模型大鼠关节滑膜组织中MMP-3与LP-PLA2的释放,减轻急性炎症反应。更多还原

【Abstract】 OBJECTIVE:To study the effects of Tongfengtai granules on MMP-3 and(LP-PL)A2 in acute gouty arthritis model rats. METHODS:Rats were randomly divided into 6 groups,namely blank control group(constant volume of normal saline), model control group(constant volume of normal saline),diclofenac group(67.5 mg/kg),Tongfengtai high-dose,medium-dose and low-dose groups(22.68,11.34 g,5.67 g/kg). The acute gouty arthritis model was induced by injection of monosodium urate solution(20 mg/ml)into the ankle of rats. The homogenate of synovium of joint were collected after 3 or 7 days treatment. ELISA was used to determine the contents of MMP-3 and LP-PLA2 in joint synovial tissue. RESULTS:After 3 days treatment,the con- tent of MMP-3 and LP-PLA2 in Tongfengtai high-dose,medium-dose group decreased significantly(P<0.05),compared with model control group. After 7 days treatment,the contents of MMP-3 and LP-PLA2 in Tongfengtai high-dose,medium-dose and low-dose groups decreased significantly(P<0.01 or P<0.05). CONCLUSIONS:Tongfengtai granules can reduce the release of MMP-3 and LP-PLA2 in acute gouty arthritis model rats,and relieve acute inflammatory reaction.更多还原