【摘要】 目的:建立测定人血清中雷公藤甲素浓度的方法。方法:采用超高效液相色谱法,内标为艾司唑仑。色谱柱为Waters Acquity C18柱,流动相为乙腈-水(30:70,V/V),流速为0.2ml/min,柱温为室温,检测波长为220nm,进样量为5μl。结果:雷公藤甲素血药浓度在13.13～840.00ng/ml范围内同雷公藤甲素与内标物峰面积比值线性关系良好;日内、日间RSD均<15%,方法回收率为88.25%～99.33%。结论:该方法快速、灵敏、准确,可用于雷公藤甲素的血药浓度监测和药动学研究。更多还原

【Abstract】 OBJECTIVE:To establish a method for determining the concentration of triptolide in human serum.METHODS:UPLC method was adopted.The column was Waters Acquity C 18 column with mobile phase consisted of acetonitrile-water(30:70,V/V)at flow rate of 0.2 ml/min using estazolam as internal standard.The temperature of column was set at room temperature and the detection wavelength was set at 220 nm.The injection volume was 5 μ l.RESULTS:The linear ranges of triptolide were 13.13-840.00 ng/ml,and RSD of intra-day and inter-day was lower than 15%.The recoveries were 88.25%-99.33%.CONCLUSION:This method is rapid,sensitive and accurate,and can be used for pharmacokinetic study and serum concentration monitoring of triptolide.更多还原