【摘要】 目的:建立同时测定舒眠胶囊中酸枣仁皂苷A和槲皮苷含量的方法。方法:采用高效液相色谱法。色谱柱为Diamonsil C18反相柱,柱温为30℃,流动相为甲醇-乙腈-缓冲液(20:30:50,V/V/V),流速为1.5ml/min,检测波长为256nm,进样量为20μl。结果:酸枣仁皂苷A、槲皮苷检测质量浓度分别在10.168～101.68mg/L(r=0.9998)、9.285～92.85mg/L(r=0.9999)范围内与峰面积积分值呈良好的线性关系;平均加样回收率分别为99.7%、99.5%,RSD分别为1.98%、2.21%(n=6)。结论:该方法简便,结果准确,重复性及回收率均理想,可用于舒眠胶囊的质量控制。更多还原

【Abstract】 OBJECTIVE:To establish a method for simultaneous determination of berberine jujuboside A and quercitroside in Shumian capsules.METHODS:HPLC method was adopted.A Diamonsil C18 reversed-phase column was used with mobile phase consisted of methanol-acetonitrile-buffer solution(20:30:50,V/V/V)at the flow rate of 1.5 ml/min.The detection wavelength was set at 256 nm and injection volume was 20 μl.RESULTS:The linear ranges of jujuboside A and quercitroside were 10.168-101.68 mg/L(r=0.999 8)and 9.285-92.85 mg/L(r=0.999 9)respectively.The average recoveries were 99.7%(RSD=1.98%,n=6) and 99.5%(RSD=2.21%,n=6).CONCLUSION:The method is simple and accurate with sound reproducibility and recovery,which can be applied for the quality control of Shumian capsule.更多还原