【摘要】 目的:研究芜菁不同提取物对糖尿病模型小鼠的降血糖作用。方法:给予小鼠腹腔注射链脲佐菌素复制糖尿病模型,尾静脉采血测空腹血糖,以血糖>11.1mmol/L为模型复制成功。实验分为空白对照(等容生理盐水)、模型(等容生理盐水)、二甲双胍(0.2g/kg)组和芜菁水提物高、低剂量(40、20g/kg),芜菁醇提物高、低剂量(40、20g/kg),芜菁醚提物高、低剂量(40、20g/kg)组。灌胃给药,每天1次,连续14d,实验期间分别于复制模型前、后,用药7、14d后剪尾取血检测血糖。末次给药结束后,小鼠摘眼球取血,分离血清测定超氧化物歧化酶(SOD)和丙二醛(MDA)水平,剖取新鲜的胸腺、脾脏,称质量,计算脏器指数。结果:与模型组比较,用药14d后,芜菁各提取物组小鼠血糖值显著降低(P<0.01或P<0.05);芜菁水提物高、低剂量组和芜菁醇提物高剂量组SOD活性显著增强(P<0.01或P<0.05);芜菁各提取物组MDA含量显著减少(P<0.05);除芜菁醇提物低剂量组外,各用药组胸腺指数、脾脏指数显著升高(P<0.01或P<0.05)。结论:高原植物芜菁对糖尿病模型小鼠有较明显降血糖作用。更多还原

【Abstract】 OBJECTIVE:To study the hypoglycemic effect of different extracts of Brassica rapa on diabetic model mice.METHODS:Diabetic mice model was induced by intraperitoneal injection of streptozotocin.Blood glucose monitoring devices was used to measure FPG through tail vein blood,setting blood glucose>11.1 mmol/L for the successful model mice.The selected mice were divided into 9 groups,i.e.blank control group(constant volume of normal saline),model group(constant volume of normal saline),metformin hydrochloride group(0.2 g/kg),B.rapa water extract high-dose and low-dose groups(40,20 g/kg),B.rapa ethanol extract high-dose and low-dose groups(40,20 g/kg)and B.rapa ether extract high-dose and low-dose groups(40,20 g/kg).They were given medicine intragastrically once a day for continuous 14 d.The tails blood glucose was determined before modeling,3 d after modeling,7 d and 14 d after treatment.After the experiment,mice eyeball blood serum was collected and separated,and SOD and MDA levels were determined.Fresh thymus and spleen were taken and weighed,and organ index was calculated.RESULTS:Compared with model group,after 14 d treatment,the blood glucose level of B.rapa extract groups decreased significantly(P<0.01 or P<0.05);the activity of SOD was improved significantly in B.rapa water extract high-dose and low-dose groups and B.rapa ethanol extract high-dose group(P<0.01 or P<0.05);MDA content decreased significantly in B.rapa extract groups;except for low-dose of B.rapa ethanol extract group,thymus index and spleen index of them increased significantly(P< 0.01 or P<0.05).CONCLUSION:Highland plant B.rapa have obvious hypoglycemic effect on diabetic mice caused by streptozotocin.更多还原