【摘要】 目的:建立一种快速、灵敏地测定芦丁含量的新方法。方法:利用在碱性条件下,芦丁对鲁米诺-高锰酸钾体系有强烈的增强作用的原理,结合流动注射技术,建立了流动注射化学发光法检测芦丁的新方法;反应试剂的浓度分别为:鲁米诺(2.0×10-5mol/L)、高锰酸钾(8.0×10-5mol/L)、氢氧化钠溶液(0.05mol/L),主泵(P1)、副泵(P2)流速分别为2.12、3.45ml/min,光电倍增管负高压为600V,采样时间为15s。结果:芦丁的检测质量浓度线性范围为2.0×10-9～2.0×10-7g/m(lr=0.9991),检出限为8.3×10-10g/ml,平均加样回收率为101.57%,RSD=1.69%(n=3)。结论:该方法简单、快速、灵敏性高,为芦丁药物的质量控制和体内分析提供了一种新的检测方法。更多还原

【Abstract】 OBJECTIVE:To establish a novel,rapid and sensitive method for the determination of rutin.METHODS:Rutin was found to enhance the chemiluminescence(CL)signal of luminol-potassium hypermanganate system in alkaline medium.Based on flow injection(FI),a new FI-CL method was developed for the determination of rutin.The concentrations of reagents were luminol(2.0×10-5 mol/L),potassium hypermanganate(8.0×10-5 mol/L),sodium hydrate(0.05 mol/L);the flow rates of pumps were set at 2.12 ml/min(P1)and 3.45 ml/min(P2)with negative high voltage of 600 V and sampling time of 15 s.RESULTS:The linear range of rutin was 2.0×10-9-2.0×10-7 g/ml(r=0.999 1)with average recovery of 101.57%(RSD=1.69,n=3).The detection limit was 8.3×10-10 g/ml.CONCLUSIONS:The method is convenient,rapid and sensitive,and can provide a new method for the quality control and product detection of rutin.更多还原