【摘要】 为研究鸭坦布苏病毒(DTMUV)Du/CH/LSD/110128在鸭胚成纤维细胞(DEF)的适应性及变异情况,本研究将Du/CH/LSD/110128接种DEF,测定各代病毒的TCID50,表明DTMUV分离株已适应DEF,并在72 h产生明显的细胞病变(CPE),自F8代起病毒适应DEF,其F10代病毒滴度为104.4TCID50/0.1 mL左右。对细胞适应毒F10代的DTMUV进行编码区基因测序并分析,结果表明Du/CH/LSD/110128细胞适应毒与DTMUV参考病毒株同源性在98%以上;其推导氨基酸序列与亲本病毒氨基酸序列差异不显著,仅存在10个氨基酸差异位点,主要在NS1和NS3蛋白,与鸡源、鸭源及鸽源病毒分别具有21个、14个、16个差异位点,这些差异位点主要存在于E蛋白、NS1蛋白和NS5蛋白。更多还原

【Abstract】 Duck Tembusu virus(DTMUV) is newly emerged virus in China which causes serious loss in duck industry.To investigate the characterization of DTMUV Du/CH/LSD/110128 in the primary duck embryo fibroblast(DEF) and nucleotide mutations,the virus was passed in the DEF and the viral encoding sequence was analyzed.The results showed that the virus was adapted to the DEF after 8 passages(F8) with stable virus titer of about 104.4TCID50/0.1 mL.In addition,the encoding sequence of the adapted virus at F10 was sequenced,and the alignment analysis showed that the cell-adapted virus with other reference strains shared more than 98% homologous.Ten amino acids mutations of F10 were detected in NS1 and NS3 proteins which were different with parental virus.However,21,14 and 16 amino acids mutations were found in F10 compared with the DTMUVs isolated from chickens,ducks and pigeons in protein E,NS1 and NS5,respectively.更多还原