Objective To study the resistance variety of Staphylococcus aureus which preserved in the microbial preservation tube with repeated freeze thaw to chlorine disinfectant. Methods The suspension quantitative germicidal test and colony morphology observation were used to observe the resistance of Staphylococcus aureus with freeze-thaw cycle. Results Compared with Staphylococcus aureus on the 1st china bead of microbial preservation tube,the 25th' color was obviously dim and whitening,and the live bacteria amou...