【摘要】 目的通过观察舒芬太尼后处理对大鼠心肌缺血再灌注中IL-6、IL-10的影响,探讨舒芬太尼后处理对心肌的保护作用。方法 40只Wistar大鼠随即分为假手术组(A组):只穿线,不结扎;缺血再灌注组(B组):缺血30min后,再灌注120min;缺血后处理组(C组):缺血30min末行缺血30s,再灌注30s,重复3次,再灌注120min;舒芬太尼后处理组(D组):缺血30min再灌注前5min静脉滴注舒芬太尼0.1μg/kg,再灌注120min。结扎大鼠心脏冠状动脉左前降支制备心肌缺血再灌注损伤模型,用ELISA方法观察各组在各采血时间点血清中IL-6、IL-10的含量,并计算心肌梗死面积。结果与假手术组相比,缺血再灌注组IL-6、IL-10含量均增高;与缺血再灌注组相比,缺血后处理组IL-6含量降低,IL-10含量增高;与缺血后处理组相比,舒芬太尼后处理组IL-6含量降低,IL-10含量增高,且4组含量数据差异均有统计学意义(P<0.05)。结论舒芬太尼后处理对大鼠心肌缺血再灌注具有保护作用,舒芬太尼后处理可能通过调控对大鼠心肌缺血再灌注血清中的IL-6、IL-10含量来发挥对心肌细胞的保护作用。更多还原

【Abstract】 Objective By observe the impact of sufentanil post-conditioning on myocardial ischemia reperfusion injury(I/RI) of IL-6,IL-10,to investigate the protective effect of sufentanil on myocardial ischemia reperfusion.Methods Forty Wistar rats were randomly divided into sham group(group A):only thread,without ligation;ischemia-reperfusion group(group B):120 min of reperfusion after 30 min of ischemia;ischemic post-conditioning group(group C):ischemia 30 min line at the end of the 30 s,reperfusion 30 s,repeat 3 times,120 min of reperfusion;sufentanil post-conditioning group(group D):5 min before 30 min of ischemia reperfusion intravenous drip sufentanil 0.1 μg/kg,120 min of reperfusion.Ligation rat heart preparation of the left anterior descending coronary artery myocardial ischemia-reperfusion injury model,observed the content of IL-6,IL-10 in serum at every blood taking time point by ELISA,and calculate the size of myocardial infarction.Results Compared with group A,the content of IL-6,IL-10 were increased in group B;Compared with group B,the content of IL-6 were decreased,and whether the content of IL-10 were increased in group C;Compared with group C,the content of IL-6 were decreased,and the content of IL-10 were increased in group D;And the data were statistically significant differences(P < 0.05).Conclusion Sufentanil post-conditioning plays a protective role on rat myocardial ischemia reperfusion injury,sufentanil post-conditioning may protect myocardial cell by regulating the content of IL-6,IL-10 in serum after the myocardial ischemia reperfusion.更多还原