【摘要】 目的观察二甲双胍对3T3-L1脂肪细胞瘦素、肿瘤坏死因子-α(TNF-α)表达与分泌量的影响,探讨二甲双胍降低体重、改善脂代谢的作用机制。方法 3T3-L1前脂肪细胞分化成熟后分别予不同浓度及作用时间的二甲双胍干预,采用RT-PCR法检测细胞内瘦素、TNF-αmRNA的表达,ELISA法测定培养基内瘦素、TNF-α的分泌量。结果二甲双胍抑制3T3-L1脂肪细胞瘦素、TNF-α的表达与分泌,呈时间与剂量依赖性。结论二甲双胍抗肥胖,改善脂代谢的作用可能与改善瘦素、TNF-α抵抗状态有关。更多还原

【Abstract】 Objective To observe the effects of metformin on the expression and secretion of leptin and tumor necrosis factor(TNF-α) in 3T3-L1 adipocytes,and to explore its mechanism in anti-obesity and lipid metabolism improvement.Methods The 3T3-L1 preadipocytes were cultured and differentiated into adipocytes,then incubated with metformin at different concentrations and durations.Leptin and TNF-α mRNA expressions were assayed by RT-PCR.The supernate contents of leptin and TNF-α were detected by ELISA.Results Metformin functioned to inhibit the leptin and TNF-α mRNA expressions and secretions in 3T3-L1 adipocytes in a concentration-and time-dependent manner.Conclusion The function of metformin in anti-obesity and lipid metabolism improvement may be related with its improvement in leptin and TNF-α sensitivity.更多还原