【摘要】 为探讨雌激素对大鼠下丘脑中凋亡相关蛋白Bcl-2和Bax表达的调控机制,采用免疫组织化学超敏SP法检测了SD大鼠摘除卵巢及外源性17β-雌二醇治疗后下丘脑中Bcl-2和Bax蛋白表达的变化。结果显示,Bcl-2和Bax蛋白在下丘脑中广泛分布,主要分布于下丘脑背内侧核、下丘脑腹内侧核、下丘脑弓状核、下丘脑室旁核和下丘脑视上核;Bcl-2和Bax在细胞中主要分布于胞膜、胞浆及核膜中,少量分布于突起中。切除卵巢后Bcl-2阳性细胞数及其表达强度出现不同程度下降,Bax蛋白表达出现不同程度上升;而注射17β-雌二醇后上述变化逆转。表明雌激素能够不同程度地下调下丘脑中Bax蛋白的表达,上调Bcl-2蛋白的表达,从而对下丘脑神经元起到保护作用。更多还原

【Abstract】 In order to elucidate the regulating mechanism of estrogen on expression of Bcl-2 and Bax in rat hypothalamus,the expression of Bcl-2 and Bax in the hypothalamus of SD rats after ovariectomized and treated with 17β-estradiol was detected by Streptavidin-Peroxidase(SP) immunohistochemistry.The results showed that Bcl-2 and Bax were expressed extensively in the hypothalamus,mainly in the nucleus dorsomedial hypothalamus,nucleus ventromedial hypothalamus,nucleus arcuatus hypothalamus,nucleus paraventricular hypothalamus and nucleus supraopticus hypothalamus.Bax and Bcl-2 mainly existed in the plasma,cytoplasmic membrane and nuclear membrane,and also observed in the neurites.In an ovariectomized group,the expression of Bcl-2 decreased,while the expression of Bax increased.After 17β-estradiol treated,Bcl-2 expressed stronger and Bax expression were reduced.All the results showed that estrogen could prevent the hypothalamus neurons from apoptosis by down-regulating the expression of Bax and up-regulating Bcl-2 at different levels.更多还原