【摘要】 为快速诊断鸡艾美耳球虫感染,基于ITS1序列建立了鸡艾美耳球虫套式PCR鉴别检测方法。该方法实现了对鸡柔嫩艾美耳球虫、堆型艾美耳球虫、早熟艾美耳球虫、和缓艾美耳球虫、毒害艾美耳球虫和布氏艾美耳球虫间的快速鉴别。测序结果和序列比对分析显示ITS1序列具有良好的种特异性,可用于艾美耳球虫属下各虫种的鉴别诊断和分类学研究,基于ITS1序列建立的套式PCR方法为艾美耳球虫鉴别诊断和分子流行病学研究提供了新的工具。更多还原

【Abstract】 To diagnose coccidiosis of chicken in a fast manner,a nested-PCR assay on the basis of the ribosomal internal transcribed spacer 1(ITS1) sequence was established to discriminate six chicken Eimeria species:E.tenella,E.mitis,E.necatrix,E.acervulina,E.praecox,and E.brunetti. Sequencing of PCR amplicons combined with the subsequent sequence analysis indicated ITS1 was able to serve as a species-specific diagnostic marker for the taxonomic research of Eimeria.This study provided a novel tool for the rapid discrimination of Eimeria species,which may be helpful in Eimeria epidemiological investigation.更多还原