【摘要】 目的:研究山杏种皮提取物总多酚含量及其体外抗氧化活性。方法:选用体积分数80%的乙醇为溶剂,采用超声辅助法制备山杏种皮提取物。采用Folin-酚试剂法测定山杏种皮提取物的总多酚含量。采用FRAP法测定山杏种皮提取物的总抗氧化能力。以2,6-二叔丁基对甲酚(BHT)、没食子酸丙酯(PG)为对照样品,分别测定山杏种皮提取物对1,1-二苯基-2-三硝基苯肼自由基(DPPH·)、超氧阴离子自由基(O2·軈)、羟自由基(·OH)的清除能力、对β-胡萝卜素/亚油酸体系的抑制能力及其还原力。结果:山杏种皮提取物中总多酚含量为(186.7±15.6)mg/g,其FRAP值为(3.715±0.012)mmol/g;对DPPH·、O2·軈、·OH具有清除作用,清除率分别为80%、97%、98%;对β-胡萝卜素/亚油酸体系具有明显的抑制作用。结论:山杏种皮提取物具有较强的抗氧化能力。更多还原

【Abstract】 Objective: the study was purported to research on the total polyphenol content and in vitro activity of testa extract of wild apricot.Method: testa extract of wild apricot was obtained by ultrasonic-assisted extraction with 80%(V/V) ethanol and its total polyphenol content and total anti-oxidative capacity were assayed with Folin’s reagent and FRAP,respectively.The capacities of test extract of wild apricot eliminating superoxide anion radical(O2′),hydroxyl radical(·OH) and 1,1-Diphenyl-2-picrylhydrazyl radical 2,2-Diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl(DPPH·) and the capacities of the testa extract inhibiting the β-carotene/linoleic acid system and reducing were assayed with 2,6-Ditert-butyl-4-methylphenol(BHT) and proply gallate(PG) as its controls.Results: the testa extract had a total polyphenol content of(186.7±15.6) mg/g and a FRADP value of(3.715±0.012) mmol/g,and capable of eliminating superoxide anion radical(O2′、),hydroxyl radical(·OH) and diphenyl bitter acyl radical(DPPH·) at the percentages of 80%,97% and 98%,respectively and evidently inhibiting the β-carotene/linoleic acid system.Conclusion: testa extract of wild apricot had relatively strong anti-oxidative capacity.更多还原