【摘要】 在粳稻嘉花1号水稻品种经60 Coγ辐照后代中筛选到一个幼苗叶色温敏感突变体tsl11。与野生型嘉花1号相比,该突变体在20℃下,幼苗叶色呈黄色;叶绿素a、叶绿素b和类胡萝卜素含量分别下降94.6%、88.2%和91.5%;且电镜结果显示叶绿体内部结构不完整。而在高温(32℃)条件下突变体的叶色表型、光合色素含量和叶绿体发育与野生型基本一致,显示出明显的温度敏感性。遗传分析表明,该突变体叶色性状受1对隐性核基因控制,暂命名为tsl11(thermo-sensitive leaf-color11)。以该突变体与籼稻培矮64S杂交,构建4928株突变型F2,利用SSR和InDel分子标记将tsl11基因定位于水稻第11染色体上的ID14506与ID14653之间的147kb的区间内。更多还原

【Abstract】 A thermo-sensitive leaf-color mutant,tsl11,was selected from the progeny of the japonica rice variety Jiahua 1 by 60Co γ-radiation.Compared with the wild-type parent Jiahua 1 at 20℃,the mutant was characterized yellow-seedling,decreased contents of chlorophyll a,chlorophyll b and carotenoid by 94.6%,88.2% and 91.5%,respectively.Simultaneously,the result of electron microscopic observation revealed that the structure of chloroplasts in the tsl11 was imperfect seriously.However,the mutant was similar to wild type at high temperatures(32℃).These results indicated that the mutant was obviously thermo-sensitive.Genetic analysis showed that the mutant trait was controlled by a single nuclear recessive gene,tentatively designated as tsl11.Moreover,the mapping population of 4928 F2 mutant individuals derived from Pei’ai 64S/tsl11 were constructed,then using SSR and InDel markers,the tsl11 gene was finally mapped to a region of 147 kb between ID14506 and ID14653 on chromosome 11.更多还原