【摘要】 目的探讨人类-β防御素-1、-2、-3mRNA(HBD-1,HBD-2,HBD-3)在非淋病奈瑟菌性宫颈炎中的表达及临床意义。方法 2010年4月至2011年5月在新疆医科大学第一附属医院选择非淋病奈瑟菌性宫颈炎72例,正常对照组64例,采用逆转录PCR(RT-PCR)技术检测宫颈组织中HBD-1、-2、-3mRNA的表达水平。结果对照组中均能检测到HBD-1、-2、-3mRNA的表达(1.12±0.41,1.08±0.31,1.04±0.30),3种防御素表达水平两两比较差异无统计学意义(P>0.05)。观察组中HBD-2,HBD-3(1.25±0.38,1.26±0.35)的表达水平明显高于对照组,分别比较差异有统计学意义(t=2.651,-3.705,P<0.009,0.000),而HBD-1在观察组表达水平(1.22±0.45)与对照组(1.12±0.41)比较,差异无统计学意义(P>0.05)。观察组及对照组宫颈组织中HBD-2mRNA的表达水平与HBD-3mRNA的表达水平均呈正相关关系(r=0.527,0.304,P=0.000,0.016),HBD-1mRNA表达水平与HBD-2、-3mRNA表达水平均无相关性(观察组r=0.253,0.267,P=0.056,0.051;对照组r=-0.018,-0.093,P=0.892,0.473)。结论人类-β防御素-2、-3mRNA在炎症发生时表达增强,两者相互促进对女性宫颈感染局部免疫防御发挥了重要作用,为今后感染治疗提供了新思路。更多还原

【Abstract】 Objective To investigate expression level and clinical significance of human beta-defensin-1、-2、-3 mRNA in nongonococcal cervicitis . Methods 72 women with nongonococcal cervicitis and 64 control group were studied in April 2010 to May 2011 at the First Affiliated Hospital of Xinjiang Medical University. The expression level of human beta-defensin-1、-2、-3 mRNA ( HBD-1、-2、-3 ) in cervical tissue was detected by reverse transcription-polymerase chain reaction ( RT-PCR) . Results The expression of HBD-1,HBD-2,HBD-3( 1. 12 ± 0. 41,1. 08 ± 0. 31,1. 04 ± 0. 30) in cervical tissue of control group all could be found and compared with each other respectively no significance( all P > 0. 05) . But the expression level of HBD-2,HBD-3 ( 1. 25 ± 0. 38,1. 26 ± 0. 35) in cervical tissue of case group were more higher than normal group ( t = 2. 651,3. 705,P < 0. 009,0. 000) . There was no difference ( P > 0. 05) in expression level of HBD1 between case( 1. 22 ± 0. 45) and normal group ( 1. 12 ± 0. 41 ) . In the case group and control group,there were positive correlation between HBD-2 and HBD-3( r = 0. 527,0. 304,P = 0. 000,0. 016) ,while HBD-1 were compared with HBD-2 and HBD-3 no correlation( case group r = 0. 253,0. 267,P = 0. 056,0. 051; control group r = 0. 018,0. 093,P = 0. 892,0. 473) respectively. Conclusion The expression of human beta-defensin-2、-3 mRNA are increase with inflectional cervices . They are strengthened through each other and play important roles in local cervical immunological defence,which provide new way of treatment.更多还原