【摘要】 参照Zadori等发表的鹅细小病毒(Goose parvovirus,GPV)B株基因序列,设计合成了一对引物,扩增GPVVP3基因。将该基因克隆于转移质粒载体pFastBac/HBM-TOPO中,获得重组转移质粒pFastBac/HBM-TOPO-VP3,并将其转化入DH10Bac细胞中,得到重组穿梭质粒Bacmid-VP3,再将其转染昆虫细胞Sf9,获得重组杆状病毒rBacmid-VP3,经PCR鉴定证实目的基因正确插入到杆状病毒基因组中。更多还原

【Abstract】 A pair of specific primers was designed according to the gene sequence of goose parvovirus (GPV) B strain,and VP3 gene of GPV was amplified by PCR. The PCR product was cloned into the transfer vector pFastBac/HBM-TOPO to obtain recombinant transfer plasmid pFastBac/HBM-TOPO-VP3 and then pFastBac/HBM-TOPO-VP3 was transformed into DH10Bac-host cells to produce recombinant shuttle plasmids Bacmid-VP3,which was transfected into Sf9 insect cells. PCR identification proved that VP3 gene were correctly inserted into baculovirus genome.更多还原