【摘要】 目的探讨兰州市汉族人群护骨素(OPG)基因多态性与老年慢性阻塞性肺疾病(COPD)患者骨质疏松遗传易感性的关系。方法选择老年COPD患者178例和老年健康组对照195例,测定肺功能(FEV1/FVC、FEV1%pred)、体重指数、血钙、血磷、血碱性磷酸酶,用双能X射线骨密度测定仪测定受试者股骨颈和腰椎(L1～L4)骨密度,应用聚合酶链反应限制性片段长度多态性方法测定OPG基因启动子区G209A与T245G基因型,并分析其与股骨颈、腰椎骨密度及骨代谢的关系。结果 COPD组OPG基因启动子区G209A与T245G基因的AAGG、GATG、GGTT三种基因型分布频率分别为2.5%、27.2%和72.3%,对照组三种基因型分布频率分别为2.2%、26.9%和70.9%,两组基因型分布差异无统计学意义(P>0.05)。COPD组和对照组不同基因型亚组的体重指数、血钙、血磷、血碱性磷酸酶及骨密度差异均无统计学意义(P>0.05)。COPD不同骨量患者OPG基因型分布差异亦无统计学意义(P>0.05)。结论兰州市老年汉族COPD患者OPG基因启动子区G209A与T245G基因多态性与患者肺功能下降、骨密度降低及骨代谢水平无显著相关性,可能不是COPD患者骨质疏松的易感位点。更多还原

【Abstract】 Objective To investigate the association between the genetic polymorphisms of osteoprotegerin gene and bone mineral density(BMD) in elderly patients with chronic obstructive pulmonary disease(COPD).Methods 178 elderly COPD patients admitted in respiratory department between January 2008 and December 2009 were recruited as a COPD group.195 elderly healthy subjects without COPD were recruited as a control group.The subjects were all chosen from the Han population in Lanzhou city,Gansu province.Pulmonary function(FEV1/FVC,FEV1%pred),body mass index(BMI),serum calcium(Ca),serum phosphate(P),and alkaline phosphatase(ALP) were determined in all subjects.The OPG gene polymorphisms were analyzed by polymerase chain reaction and restriction fragment length polymorphism(PCR-RFLP).BMD was examined by dual-energy X-ray absorptiometry.Results In the COPD group,the distribution frequency of AAGG,GATA,and GGTT in OPG HTT gene-linked polymorphic region G209A and T245G were 2.5%,27.2%,and 72.3%,respectively,which in the control group were 2.2%,26.9%,and 70.9%,respectively.The genotype distribution difference of two groups had no statistical significance(P>0.05).There were also no statistical differences in BMI,serum Ca,serum P,serum ALP or BMD between different genotype subgroups in two groups(P>0.05).In the COPD group,the genotype distribution had no statistical significance between different BMD subgroups(P>0.05).Conclusion In the elderly patients with COPD from Han population at Lanzhou city,OPG HTT gene-linked polymorphic region and T245G gene polymorphism have no significant correlation with reduced lung function,reduced BMD and bone metabolism which are not likely to be susceptibility loci for osteoporosis in COPD patients.更多还原