【摘要】 研究邻苯二甲酸丁基苄酯(BBP)暴露对小鼠脑组织的氧化损伤和对小鼠学习与记忆能力的影响.24只雄性昆明小鼠随机分为4组,每组6只,分别暴露于0,50,250,1250mg/kg的BBP,经口灌胃染毒14d.染毒期间同时进行Morris水迷宫实验,以检测小鼠的学习与记忆能力的改变.实验进行第15d,处死实验动物,取出脑、肝、肾组织,检测ROS水平及MDA和GSH含量.实验结果显示,1250mg/kg浓度BBP染毒组小鼠的学习和记忆能力与对照组相比显著下降(P<0.05);随着BBP染毒浓度的升高,小鼠脑、肝、肾组织中的ROS水平、MDA含量逐渐上升,GSH含量逐渐降低;且在1250mg/kg浓度时与对照组相比具有显著性差异(P<0.05).BBP的暴露可以影响小鼠学习与记忆能力,并对其脑、肝、肾组织产生氧化损伤.更多还原

【Abstract】 To investigate the oxidative damage of brain and the effects on learning and memory ability induced by butylbenzyl phthalate(BBP) in mice.Twenty-four male Kun Ming(KM) mice were randomly assigned into 4 groups(n=6),exposed to different concentrations of BBP(0,50,250 and 1250 mg/kg) by oral gavage for fourteen days.During the exposure period,the Morris water maze was used to test the learning and memory abilities.After Morris water maze test the experimental animals were ended lives,and then the reactive oxygen species(ROS) level,malondialdehyde(MDA) and glutathione(GSH) contents of brains,liver and kidney were measured.BBP caused significant differences in the abilities of learning and memory at 1250mg/kg by the Morris water maze(P<0.05).Along with the increase of BBP concentrations,increased ROS level,MDA content,and reduced GSH content were observed.BBP could induce oxidative damage in brains,liver and kidney of mice,and have negative effects on the abilities of learning and memory.更多还原