【摘要】 目的了解伯氏疟原虫(Plasmodium berghei NK65,P.b NK65)感染幼年和中年C57BL/6小鼠免疫反应特点。方法 P.b NK65感染3周龄幼年和8月龄中年C57BL/6小鼠,采用流式细胞分析技术检测不同年龄组小鼠脾细胞悬液中髓样树突状细胞(dendritic cells,DCs)数量,表面表达MHCⅡ类分子和CD86分子的DCs数量以及分泌IgG抗体的B细胞数量;ELISA方法检测脾细胞培养上清中IFN-γ和IL-4水平。结果 3周小鼠DCs数量[(40.08±3.54)×105]在感染后5 d明显升高,8月龄鼠在感染后5 d[(103.47±11.07)×105]及8 d[(104.04±1.92)×105]明显升高;3周鼠表面表达MHCⅡ类分子和CD86分子的DCs数量在感染后明显升高,8月龄鼠在感染后8 d表达MHCⅡ类分子[(239.40±16.32)×105]和CD86分子[(148.48±14.84)×105]数量明显高于3周鼠(P<0.05);3周鼠[(251.01±34.02)pg/mL]和8月龄鼠[(584.17±54.03)pg/mL]IFN-γ水平在感染后5 d均明显升高,2组比较差异有统计学意义(P<0.05)。结论 3周和8月龄C57BL/6小鼠感染P.b NK65后,其细胞免疫和体液免疫存在明显差异。更多还原

【Abstract】 Objective To investigate the characteristics of immune response to infection of Plasmodium berghei NK65(P. b NK65) in young and middle aged C57BL/6 mice. Methods Three weeks and 8 months aged C57BL/6 mice were infected by P. b NK65 parasitized erythrocytes. Flow cytometry was applied to quantitatively analyze myeloid dendritic cells(DCs) and DCs expressing major histocompatibility complex class Ⅱ(MHC Ⅱ) or CD86. The levels of interferon gamma(IFN-γ) and interleukin-4(IL-4) in spleen cell supermatants were dynamically detected by enzyme-linked immunosorbent assay(ELISA). Results The number of myeloid DCs(40. 08 × 105) in young aged mice significantly increased 5 days after the infection. The number of myeloid DCs 5 and 8 days after the infection was 103. 47 × 105 and 104. 04 × 105 in the middle aged mice. The numbers of DCs expressing MHC Ⅱor CD86 were 50. 16 × 105and 45. 39 × 105in young aged mice,and in middle aged mice the numbers were 239. 40 × 105 and 148. 48 × 105,which was markedly more than young aged mice. The level of IFN-γ significantly increased both in young aged(251. 01 pg/ml) and middle aged mice(584. 17 pg/ml). Conclusion The cellular and humoral immune responses to infection of P. b NK65 are different betw een the young and middle aged C57BL/6 mice.更多还原