【摘要】 目的探讨壬基酚对大鼠前列腺组织细胞凋亡及caspase-3、caspase-8和caspase-9 mRNA表达影响。方法健康清洁级Wistar雄性大鼠50只,按体重随机分为5组,分别为12.5、25、50、100 mg/kg壬基酚染毒组和对照组(橄榄油),每组10只;皮下注射染毒(1 mL/kg),每天1次,连续染毒30 d;Tunel法观察前列腺前叶组织细胞凋亡,实时荧光定量聚合酶链式反应(RT-PCR)法观察组织中caspase-3、caspase-8、caspase-9 mRNA表达。结果 12.5、25、50、100 mg/kg壬基酚染毒组大鼠前列腺细胞凋亡率分别为(3.07±1.31)%、(5.21±1.47)%、(6.31±1.29)%、(8.90±0.18)%,均低于对照组的(12.73±2.19)%(P<0.05);与对照组比较,12.5、25、50、100 mg/kg壬基酚染毒组大鼠前列腺组织细胞caspase-3、caspase-8、caspase-9 mRNA表达均降低(P<0.05)。结论壬基酚可致雄性大鼠前列腺细胞凋亡率下降,其机制可能与前列腺细胞caspase-3、caspase-8和caspase-9 mRNA表达降低有关。更多还原

【Abstract】 Objective To evaluate effects of nonylphenol(NP)on rat prostate tissue apoptosis and expressions of caspase-3,caspase-8,and caspase-9 mRNA.Methods Fifty healthy Wistar rats were randomly divided into five groups(one olive control group and 4 groups exposed to NP at the doses of 12.5,25,50,and 100 mg/kg)based on body weight.The exposure groups were admimistered 1 mg/kg of NP hypodermic injection once a day for 30 days.The prostate tissue apoptosis was observed with teminal transferase-mediated biotin dUTP nick end labeling(TUNEL)and expressions of caspase-3,caspase-8,and caspase-9mRNA in prostate tissue were detected with real-time reverse transcription PCR(RT-PCR).Results The prostate apoptosis rate in 12.5,25,50,and 100 mg/kg NP group were 3.07±1.31%,5.21±1.47%,6.31±1.29%,and 8.90±0.18%,all lower than that in the control group(12.73±2.19%)(P<0.05 for all).The expressions of caspase-3 mRNA,caspase-8 mRNA,and caspase-9 mRNA in all NP exposed groups were lower than those in the control group(P<0.05 for all).Conclusion NP decreases the apoptosis of prostate tissue in male Wistar rats,which may be one of the mechanisms for prostate hyperplasia in rats caused by NP.更多还原