【摘要】 目的探讨嗜肺军团菌活菌刺激对人外周血单个核细胞(PBMC)表达TLR2 mRNA的影响。方法采用不同浓度的嗜肺军团菌活菌悬液刺激体外培养的健康人PBMC,收集细胞运用RT-PCR方法测定TLR2 mRNA的表达水平。结果析因分析结果显示,时间的主效应有统计学意义(F=26.06,P<0.05);时间与浓度的交互作用有统计学意义(F=11.39,P<0.05);24 h时,MOI 1、MOI 10刺激组TLR2 mRNA的表达量均高于对照组(P<0.05),呈现随菌液浓度升高表达量也逐渐升高,48 h时,各组之间TLR2 mRNA的表达量差异虽无统计学意义(P>0.05),但表达量随浓度升高呈现下降趋势,72 h时,各组之间TLR2 mRNA的表达量差异有统计学意义(P<0.05),但随菌液浓度升高表达量反而降低。结论用军团菌体外刺激人PBMC,其TLR2 mRNA的表达在一定范围内呈一定的时效与量效关系,可为今后的研究提供有益的时间和剂量参考。更多还原

【Abstract】 Objective To examine the expression of TLR2 in human peripheral blood mononuclear cells(PBMCs) stimulatedby Legionella.Methods Human peripheral blood mononuclear cells(POMCs) were treated with different concentrations of Legionella(2×106/ml,2×107/ml),and real timel reverse transcriptase PCR(RT-PCR) was used to detect TLR2 mRNA expression.Results Factorial analyses showed that leading effect of time had statistical significance(F=26.06,P<0.05) and there was an interaction between the time and concentration(F=11.39,P<0.05).In 24 hours,the expressions of TLR2 mRNA of PBMCs stimulated with 2×106/ml and 2×107/ml Legionella were obviously higher than TLR2 mRNA expression in control group.In 48 hours,there was no difference between stimulated group and control group.In 72 hours,the expressions of TLR2 mRNA of PBMCs stimulated with 2×106/ml and 2×107/ml Legionella,were obviously lower than TLR2 mRNA expression in the control group.When the PBMCs were stimulated at the level of 2×106/ml and 2×107/ml Legionella,the difference in the expression of TLR2 mRNA in each period was statistically significant.Conclusion Expression of TLR2 mRNA in human peripheral blood mononuclear cells with Legionella is correlated with the time and dosage in vitro.The research provides a reference for the time and dose in the study.更多还原