【摘要】 目的观察不同浓度七氟醚对人鼻咽癌细胞株CNE2黏附能力、CD133表达的影响。方法分为四组:对照组(Control组)、1.7%七氟醚组(Treat1组)、3.4%七氟醚组(Treat2组)和5.1%七氟醚组(Treat3组)。Treat1组、Treat2组和Treat3组人鼻咽癌CNE2细胞,经1.7%、3.4%、5.1%七氟醚作用2 h、4 h、6 h。检测细胞黏附率;采用qRT-PCR法检测CD133的mRNA表达。结果与处理前比较,Treat1组、Treat2组和Treat3组处理2 h、4 h和6 h时细胞黏附率降低,CD133的mRNA及其蛋白表达下调(P<0.05)。结论七氟醚可降低人鼻咽癌细胞株CNE2的黏附能力,且呈浓度和时间依赖性,其机制可能与下调CD133的表达有关。更多还原

【Abstract】 Objective To investigate the effects of different concentrations of sevoflurane on adhesion and expression of CD133 in human nasopharyngeal carcinoma cell line CNE2. Methods The cells were randomly divided into 4 groups:control group and 3 sevoflurane groups for 2,4 and 6 h respectively. Cell adhesion rate was detected by adhesion test and the expression of CD133 mRNA and protein was determined by qRT-PCR. Results Sevoflurane significantly inhibited the cell adhesion rate and down-regulated CD133 expression in a concentration and duration of expo-sure-dependent manner. Conclusion Sevoflurane can inhibit cell adhesion through down-regulation of CD133 expres-sion.更多还原